Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose

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Title
Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose
Author(s)
Suk Weon Kim; Myung Jin Oh; Jang Ryol Liu
Bibliographic Citation
Plant Biotechnology Reports, vol. 3, no. 3, pp. 199-203
Publication Year
2009
Abstract
Rugosa rose (Rosa rugosa) is cultivated as a garden flower and an important genetic resource for the breeding of roses (R. hybrida). This study describes culture conditions for high frequency plant regeneration from zygotic embryo explants via somatic embryogenesis in rugosa rose. Mature zygotic embryo, cotyledon, and radicle explants formed embryogenic calluses at frequencies of 38, 6.7, and 8.8% when cultured on half-strength Murashige and Skoog medium (1/2MS) supplemented with 2.26, 9.05, and 9.05 μM 2,4-dichlorophenoxyacetic acid, respectively. Embryogenic calluses produced numerous somatic embryos, which then developed into plantlets on 1/2MS without growth regulators. Regenerated plantlets were grown to whole plants in a growth chamber.
Keyword
Rosa rugosaSomatic embryogenesisTissue culture
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-009-0092-9
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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