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- Establishment of high frequency plant regeneration system from leaf explants of Pinellia koreana via bulblets formation
- Myung Jin Oh; Jongmi Park; B Y Lee; P S Choi; K H Tae; Suk Weon Kim
- Bibliographic Citation
- Journal of Plant Biotechnology, vol. 36, no. 2, pp. 193-196
- Publication Year
- Pinellia koreana K-H Tae & J-H Kim is a recently discovered Korea endemic medicinal plant species whose
natural habitat is rapidly destroyed by industrial development. Described in this paper are culture conditions for high frequency
plant regeneration via bulblet formation from leaf explant cultures of P. koreana. Leaf explants formed white nodular
structures and off-white calluses at a frequency of 91.2% when cultured on MS medium supplemented with 2 mg/L BA
and 0.5 mg/L NAA. However, the frequency of white nodular structures and off-white calluses formation was slightly decreased
with an increasing concentration of NAA up to 4 mg/L, where the frequency reached 31.7%. Most petiole explants did
not form white nodular structures and off-white calluses except the combination treatment of 2 mg/L BA and 2 mg/L NAA.
Upon transfer onto MS basal medium, over 90% of nodular structures gave rise to numerous bulblets and developed into
plantlets. Plantlets regenerated from bulblets were transplanted to potting soil and grown to maturity at a survival rate of
over 95% in a growth chamber. Therefore, the in vitro plant regeneration system of P. koreana obtained in this study will
be useful for mass propagation and long-term preservation of genetic resources of P. koreana.
- Korea Soc-Assoc-Inst
- Appears in Collections:
- Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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