High frequency plant proliferation via direct fronds regeneration of Korean endemic duckweed species = 국내 자생 좀개구리밥(Lemna paucicostata) 및 개구리밥(Spirodela polyrhiza)의 엽상체 증식을 통한 기내 식물체 대량 증식체계 확립

Cited 0 time in scopus
Metadata Downloads
Title
High frequency plant proliferation via direct fronds regeneration of Korean endemic duckweed species = 국내 자생 좀개구리밥(Lemna paucicostata) 및 개구리밥(Spirodela polyrhiza)의 엽상체 증식을 통한 기내 식물체 대량 증식체계 확립
Author(s)
Myung Jin Oh; Jongmi Park; S M Ko; Jang Ryol Liu; Suk Weon Kim
Bibliographic Citation
Journal of Plant Biotechnology, vol. 36, no. 2, pp. 157-162
Publication Year
2009
Abstract
High frequency plant proliferation system via direct frond regeneration of endemic duckweed plants Lemna paucicostata and Spirodela polyrhiza was established. Fronds of L. paucicostata and S. polyrhiza were able to multiply half-strength MS basal medium without plant growth regulators. However, addition of BA at a range of 1 to 3 mg/L was more effective than high concentration of BA treatments for fronds proliferation. Also half-strength MS salts was suitable for the fronds proliferation. Increase of salts concentration had inhibitory effect on fronds proliferation. Also the frequency of callus formation from fronds of L. paucicostata was 3.3%, when they cultured onto 1/2 MS medium supplemented with 1 mg/L of BA. Similarly the frequency of callus formation from S. polyrhiza was very low. After subculture of white globular structures derived from fronds of L. paucicostata, numerous globular somatic embryos and calluses were developed onto the surface of fronds. However these somatic embryos did not fully develop into normal plants when transferred to 1/2 MS basal medium. Therefore direct frond regeneration system was more efficient for mass proliferation of L. paucicostata and S. polyrhiza. The plant regeneration system of L. paucicostata and S. polyrhiza established in this study, might be applied to mass proliferation and genetic transformation for molecular breeding.
ISSN
1229-2818
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2009.36.2.157
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.