Proteomic analysis of liver tissue from HBx-transgenic mice at early stages of hepatocarcinogenesis

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Title
Proteomic analysis of liver tissue from HBx-transgenic mice at early stages of hepatocarcinogenesis
Author(s)
Sun Young Kim; P Y Lee; H J Shin; D H Kim; Sunghyun Kang; H B Moon; S W Kang; J M Kim; Sung Goo ParkByoung Chul Park; Dae Yeul Yu; Kwang-Hee BaeSang Chul Lee
Bibliographic Citation
Proteomics, vol. 9, no. 22, pp. 5056-5066
Publication Year
2009
Abstract
The hepatitis B virus X-protein (HBx), a multifunctional viral regulator, participates in the viral life cycle and in the development of hepatocellular carcinoma (HCC). We previously reported a high incidence of HCC in transgenic mice expressing HBx. In this study, proteomic analysis was performed to identify proteins that may be involved in hepatocarcinogenesis and/or that could be utilized as early detection biomarkers for HCC. Proteins from the liver tissue of HBx-transgenic mice at early stages of carcinogenesis (dysplasia and hepatocellular adenoma) were separated by 2-DE, and quantitative changes were analyzed. A total of 22 spots displaying significant quantitative changes were identified using LC-MS/MS. In particular, several proteins involved in glucose and fatty acid metabolism, such as mitochondrial 3-ketoacyl-CoA thiolase, intestinal fatty acid-binding protein 2 and cytoplasmic malate dehydrogenase, were differentially expressed, implying that significant metabolic alterations occurred during the early stages of hepatocarcinogenesis. The results of this proteomic analysis provide insights into the mechanism of HBx-mediated hepatocarcinogenesis. Additionally, this study identifies possible therapeutic targets for HCC diagnosis and novel drug development for treatment of the disease.
Keyword
Animal proteomicsDysplasiaHepatitis B virus X-proteinHepatocellular adenomaHepatocellular carcinoma
ISSN
1615-9853
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/pmic.200800779
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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