Regulation of adipogenic differentiation by LAR tyrosine phosphatase in human mesenchymal stem cells and 3T3-L1 preadipocytes

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dc.contributor.authorWon Kon-Kim-
dc.contributor.authorHyeyun Jung-
dc.contributor.authorDo-Hyung Kim-
dc.contributor.authorEun Young Kim-
dc.contributor.authorJ W Chung-
dc.contributor.authorYeesook Cho-
dc.contributor.authorSung Goo Park-
dc.contributor.authorByoung Chul Park-
dc.contributor.authorY Ko-
dc.contributor.authorKwang-Hee Bae-
dc.contributor.authorSang Chul Lee-
dc.date.accessioned2017-04-19T09:15:31Z-
dc.date.available2017-04-19T09:15:31Z-
dc.date.issued2009-
dc.identifier.issn0021-9533-
dc.identifier.uri10.1242/jcs.053009ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9210-
dc.description.abstractMesenchymal stem cells (MSCs) are multipotent adult stem cells that can differentiate into a variety of mesodermal-lineage cells. MSCs have significant potential in tissue engineering and therapeutic applications; however, the low differentiation and proliferation efficiencies of these cells in the laboratory are fundamental obstacles to their therapeutic use, mainly owing to the lack of information on the detailed signal-transduction mechanisms of differentiation into distinct lineages. With the aid of protein-tyrosine-phosphatase profiling studies, we show that the expression of leukocyte common antigen related (LAR) tyrosine phosphatase is significantly decreased during the early adipogenic stages of MSCs. Knockdown of endogenous LAR induced a dramatic increase in adipogenic differentiation, whereas its overexpression led to decreased adipogenic differentiation in both 3T3-L1 preadipocytes and MSCs. LAR reduces tyrosine phosphorylation of the insulin receptor, in turn leading to decreased phosphorylation of the adaptor protein IRS-1 and its downstream molecule Akt (also known as PKB). We propose that LAR functions as a negative regulator of adipogenesis. Furthermore, our data support the possibility that LAR controls the balance between osteoblast and adipocyte differentiation. Overall, our findings contribute to the clarification of the mechanisms underlying LAR activity in the differentiation of MSCs and suggest that LAR is a candidate target protein for the control of stem-cell differentiation.-
dc.publisherCompany Biologists Ltd-
dc.titleRegulation of adipogenic differentiation by LAR tyrosine phosphatase in human mesenchymal stem cells and 3T3-L1 preadipocytes-
dc.title.alternativeRegulation of adipogenic differentiation by LAR tyrosine phosphatase in human mesenchymal stem cells and 3T3-L1 preadipocytes-
dc.typeArticle-
dc.citation.titleJournal of Cell Science-
dc.citation.number22-
dc.citation.endPage4167-
dc.citation.startPage4160-
dc.citation.volume122-
dc.contributor.affiliatedAuthorWon Kon-Kim-
dc.contributor.affiliatedAuthorHyeyun Jung-
dc.contributor.affiliatedAuthorDo-Hyung Kim-
dc.contributor.affiliatedAuthorEun Young Kim-
dc.contributor.affiliatedAuthorYeesook Cho-
dc.contributor.affiliatedAuthorSung Goo Park-
dc.contributor.affiliatedAuthorByoung Chul Park-
dc.contributor.affiliatedAuthorKwang-Hee Bae-
dc.contributor.affiliatedAuthorSang Chul Lee-
dc.contributor.alternativeName김원곤-
dc.contributor.alternativeName정혜윤-
dc.contributor.alternativeName김도형-
dc.contributor.alternativeName김은영-
dc.contributor.alternativeName정진웅-
dc.contributor.alternativeName조이숙-
dc.contributor.alternativeName박성구-
dc.contributor.alternativeName박병철-
dc.contributor.alternativeName고용-
dc.contributor.alternativeName배광희-
dc.contributor.alternativeName이상철-
dc.identifier.bibliographicCitationJournal of Cell Science, vol. 122, no. 22, pp. 4160-4167-
dc.identifier.doi10.1242/jcs.053009-
dc.subject.keywordAdipocyte-
dc.subject.keywordAdipogenesis-
dc.subject.keywordLeukocyte common antigenrelated-
dc.subject.keywordMesenchymal stem cells-
dc.subject.keywordOsteoblast-
dc.subject.keywordProtein tyrosine phosphatase-
dc.subject.localAdipocyte-
dc.subject.localadipocyte-
dc.subject.localAdipocytes-
dc.subject.localadipocytes-
dc.subject.localadipogenesis-
dc.subject.localADIPOGENESIS-
dc.subject.localAdipogenesis-
dc.subject.localLeukocyte common antigenrelated-
dc.subject.localMesenchymal stem cell-
dc.subject.localmesenchymal stem cells-
dc.subject.localmesenchymal stem cells (MSCs)-
dc.subject.localMesenchymal stem cells-
dc.subject.localOsteoblast-
dc.subject.localosteoblast-
dc.subject.localosteoblasts-
dc.subject.localOsteoblasts-
dc.subject.localProtein tyrosine phosphatases-
dc.subject.localProtein tyrosine phosphatase-
dc.subject.localprotein tyrosine phosphatase-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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