Synapse formation regulated by protein tyrosine phosphatase receptor T through interaction with cell adhesion molecules and Fyn = PTPRT에 의한 신경시냅스 형성 조절

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Title
Synapse formation regulated by protein tyrosine phosphatase receptor T through interaction with cell adhesion molecules and Fyn = PTPRT에 의한 신경시냅스 형성 조절
Author(s)
So Hee Lim; S K Kwon; Myung Kyu Lee; Jeong Hee MoonDae Gwin Jeong; Eun Ha Park; Seung Jun KimByoung Chul ParkSang Chul Lee; S E Ryu; Dae Yeul Yu; Bong Hyun Chung; E Kim; P K Myung; Jae-Ran Lee
Bibliographic Citation
EMBO Journal, vol. 28, no. 22, pp. 3564-3578
Publication Year
2009
Abstract
The receptor-type protein tyrosine phosphatases (RPTPs) have been linked to signal transduction, cell adhesion, and neurite extension. PTPRT/RPTP is exclusively expressed in the central nervous system and regulates synapse formation by interacting with cell adhesion molecules and Fyn protein tyrosine kinase. Overexpression of PTPRT in cultured neurons increased the number of excitatory and inhibitory synapses by recruiting neuroligins that interact with PTPRT through their ecto-domains. In contrast, knockdown of PTPRT inhibited synapse formation and withered dendrites. Incubation of cultured neurons with recombinant proteins containing the extracellular region of PTPRT reduced the number of synapses by inhibiting the interaction between ecto-domains. Synapse formation by PTPRT was inhibited by phosphorylation of tyrosine 912 within the membrane-proximal catalytic domain of PTPRT by Fyn. This tyrosine phosphorylation reduced phosphatase activity of PTPRT and reinforced homophilic interactions of PTPRT, thereby preventing the heterophilic interaction between PTPRT and neuroligins. These results suggest that brain-specific PTPRT regulates synapse formation through interaction with cell adhesion molecules, and this function and the phosphatase activity are attenuated through tyrosine phosphorylation by the synaptic tyrosine kinase Fyn.
Keyword
Cell adhesion moleculeFynPTPRTSynapse formationTyrosine phosphorylation
ISSN
0261-4189
Publisher
Wiley
DOI
http://dx.doi.org/10.1038/emboj.2009.289
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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