Development of transgenic sweet potato producing human lactoferrin = 인체 락토페린 생산 형질전환 고구마 개발

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Title
Development of transgenic sweet potato producing human lactoferrin = 인체 락토페린 생산 형질전환 고구마 개발
Author(s)
Sung Ran MinJae Wha KimWon Joong Chung; Y B Lee; Jang Ryol Liu
Bibliographic Citation
Journal of Plant Biotechnology, vol. 36, no. 3, pp. 224-229
Publication Year
2009
Abstract
Human lactoferrin is an iron-binding glycoprotein with many biological activities, including the protection against microbial and virus infection and stimulation of the immune system. We introduced a human lactoferrin (hLf) cDNA under the control of 35S promoter into sweet potato by particle bombardment. Transgenic plants were regenerated via somatic embryogenesis. Transgenic plants were produced typical tuberous roots in soil. PCR, Southern and northern analyses confirmed that the hLf cDNA was incorporated into the plant genome and was properly expressed in plants. Western blot analysis showed that the 80 kDa full length hLf protein was produced in transgenic tuberous roots. Overall results indicated that sweet potato would be an excellent host to produce human therapeutic proteins.
ISSN
1229-2818
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2009.36.3.224
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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