Psychroflexus salinarum sp. nov., isolated from a marine solar saltern

Abstract

A Gram-negative, non-motile and rod-shaped bacterial strain, ISL-14T, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by a polyphasic study. Strain ISL-14T grew optimally at pH 7.0-8.0, 30 °C and in the presence of approximately 2% (w/v) NaCl. It contained MK-6 as the predominant menaquinone and anteiso-C15:0, iso-C15:0 and iso-C16:0 3-OH as the major fatty acids. The DNA G+C content was 38.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-14T belonged to the genus Psychroflexus. The levels of similarity between the 16S rRNA gene sequence of strain ISL-14T and those of the type strains of recognized Psychroflexus species were 95.8-96.8%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain ISL-14T represents a novel species within the genus Psychroflexus, for which the name Psychroflexus salinarum sp. nov. is proposed. The type strain is ISL-14T (=KCTC 22483T=CCUG 56752T).