Production of fibrinolytic enzyme in plastid-transformed tobacco plants

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Title
Production of fibrinolytic enzyme in plastid-transformed tobacco plants
Author(s)
S M Ko; B H Yoo; J M Lim; K H Oh; J I Liu; Suk Weon Kim; Jang Ryol Liu; K S Choi; E S Yoon
Bibliographic Citation
Plant Molecular Biology Reporter, vol. 27, no. 4, pp. 448-453
Publication Year
2009
Abstract
The earthworm fibrinolytic enzyme, which belongs to a group of serine proteases with strong fibrinolytic activity, has been used as an oral drug for prevention and treatment of thrombosis in East Asia. Fibrizyme is a fibrinolytic enzyme isolated from the earthworm Eisenia andrei. Here we report genetic engineering of tobacco plastids with stable integration of the fibrizyme gene into the tobacco chloroplast genome. A plastid transformation vector was constructed by introducing various regulatory elements into fibrizyme cDNA. This vector was delivered by particle bombardment into tobacco leaf explants and plastid-transformed plants were subsequently regenerated into whole plants through several rounds of selection. We confirmed stable integration of the fibrizyme gene into the tobacco plastid genome by PCR and Southern blot analyses. Northern and Western blot analyses revealed that mRNA and protein of recombinant fibrizyme were highly expressed in transformed tobacco plants.
Keyword
BiopharmaceuticalChloroplast transformationFibrinolytic enzymeGenetically modified crops
ISSN
0735-9640
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11105-009-0113-4
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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