Development of gradient centrifugal partition chromatography method and its application for the isolation of 3,5-dimethoxyphenanthrene-2,7-diol and batatasin-I from Dioscorea opposita

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dc.contributor.authorK D Yoon-
dc.contributor.authorM H Yang-
dc.contributor.authorYoung Won Chin-
dc.contributor.authorY Kim-
dc.contributor.authorH R Kim-
dc.contributor.authorK Choi-
dc.contributor.authorJ H Park-
dc.contributor.authorJ Kim-
dc.date.accessioned2017-04-19T09:16:14Z-
dc.date.available2017-04-19T09:16:14Z-
dc.date.issued2009-
dc.identifier.issnI0000147-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9279-
dc.description.abstractGradient centrifugal partition chromatography (GCPC) method was developed and applied to isolate 3,5-dimethoxyphenanthrene-2,7-diol (DMP) and batatasin-I (BA-I) from the dichloromethane soluble extract of Dioscorea opposita. In this method, the lower phase of n-hexane-methanol-water system (HMW, 10 : 9 : 1, v/v) was used as a mobile phase A (MpA) and water was used as a mobile phase B (MpB). This gradient CPC method is comparable to that of reversed-phase HPLC method in that the stationary upper-phase of HMW (10 : 9 : 1 v/v) works as if it were reversed-phase silica gel due to its hydrophobic property, while the lower phase (MpA) and water (MpB) functioned as hydrophilic mobile phases. The initial condition of the mobile phase was 20% MpA/80% MpB and maintained for 150 min to obtain DMP (1.2 mg), and then MpA was increased up to 50% to elute BA-I (1.7 mg). The purities of DMP and BA-I were 94.1% and 98.3% with the recovery yields of 83% and 86%,respectively. Similar results were obtained by linear-gradient CPC. The CPC peak fractions were identified by comparing their retention time to those of authentic samples of DMP and BA-I and their spectroscopic data (1HNMR and 13C NMR) to those of literature values.-
dc.publisherKorean Society of Pharmacognosyko
dc.titleDevelopment of gradient centrifugal partition chromatography method and its application for the isolation of 3,5-dimethoxyphenanthrene-2,7-diol and batatasin-I from Dioscorea opposita-
dc.title.alternativeDevelopment of gradient centrifugal partition chromatography method and its application for the isolation of 3,5-dimethoxyphenanthrene-2,7-diol and batatasin-I from Dioscorea opposita-
dc.typeArticle-
dc.citation.titleNatural Products Sciences-
dc.citation.number3-
dc.citation.endPage150-
dc.citation.startPage144-
dc.citation.volume15-
dc.contributor.alternativeName윤기동-
dc.contributor.alternativeName양민혜-
dc.contributor.alternativeName진영원-
dc.contributor.alternativeName김연준-
dc.contributor.alternativeName김혜령-
dc.contributor.alternativeName최기리-
dc.contributor.alternativeName박주현-
dc.contributor.alternativeName김진웅-
dc.identifier.bibliographicCitationNatural Products Sciences, vol. 15, no. 3, pp. 144-150-
dc.subject.keyword3,5-dimethoxyphenanthrene-2,7-diol-
dc.subject.keywordbatatasin-I-
dc.subject.keywordDioscorea opposita-
dc.subject.keywordGradient centrifugal partition chromatography (GCPC)-
dc.subject.keywordn-hexane-methanol-water system (HMW, 10 : 9 : 1, v/v)-
dc.subject.local3,5-dimethoxyphenanthrene-2,7-diol-
dc.subject.localbatatasin-I-
dc.subject.localDioscorea opposita-
dc.subject.localGradient centrifugal partition chromatography (GCPC)-
dc.subject.localn-hexane-methanol-water system (HMW, 10 : 9 : 1, v/v)-
dc.description.journalClassN-
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