DC Field | Value | Language |
---|---|---|
dc.contributor.author | K D Yoon | - |
dc.contributor.author | M H Yang | - |
dc.contributor.author | Young Won Chin | - |
dc.contributor.author | Y Kim | - |
dc.contributor.author | H R Kim | - |
dc.contributor.author | K Choi | - |
dc.contributor.author | J H Park | - |
dc.contributor.author | J Kim | - |
dc.date.accessioned | 2017-04-19T09:16:14Z | - |
dc.date.available | 2017-04-19T09:16:14Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | I000-0147 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9279 | - |
dc.description.abstract | Gradient centrifugal partition chromatography (GCPC) method was developed and applied to isolate 3,5-dimethoxyphenanthrene-2,7-diol (DMP) and batatasin-I (BA-I) from the dichloromethane soluble extract of Dioscorea opposita. In this method, the lower phase of n-hexane-methanol-water system (HMW, 10 : 9 : 1, v/v) was used as a mobile phase A (MpA) and water was used as a mobile phase B (MpB). This gradient CPC method is comparable to that of reversed-phase HPLC method in that the stationary upper-phase of HMW (10 : 9 : 1 v/v) works as if it were reversed-phase silica gel due to its hydrophobic property, while the lower phase (MpA) and water (MpB) functioned as hydrophilic mobile phases. The initial condition of the mobile phase was 20% MpA/80% MpB and maintained for 150 min to obtain DMP (1.2 mg), and then MpA was increased up to 50% to elute BA-I (1.7 mg). The purities of DMP and BA-I were 94.1% and 98.3% with the recovery yields of 83% and 86%,respectively. Similar results were obtained by linear-gradient CPC. The CPC peak fractions were identified by comparing their retention time to those of authentic samples of DMP and BA-I and their spectroscopic data (1HNMR and 13C NMR) to those of literature values. | - |
dc.publisher | Korean Society of Pharmacognosy | ko |
dc.title | Development of gradient centrifugal partition chromatography method and its application for the isolation of 3,5-dimethoxyphenanthrene-2,7-diol and batatasin-I from Dioscorea opposita | - |
dc.title.alternative | Development of gradient centrifugal partition chromatography method and its application for the isolation of 3,5-dimethoxyphenanthrene-2,7-diol and batatasin-I from Dioscorea opposita | - |
dc.type | Article | - |
dc.citation.title | Natural Products Sciences | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 150 | - |
dc.citation.startPage | 144 | - |
dc.citation.volume | 15 | - |
dc.contributor.affiliatedAuthor | Young Won Chin | - |
dc.contributor.alternativeName | 윤기동 | - |
dc.contributor.alternativeName | 양민혜 | - |
dc.contributor.alternativeName | 진영원 | - |
dc.contributor.alternativeName | 김연준 | - |
dc.contributor.alternativeName | 김혜령 | - |
dc.contributor.alternativeName | 최기리 | - |
dc.contributor.alternativeName | 박주현 | - |
dc.contributor.alternativeName | 김진웅 | - |
dc.identifier.bibliographicCitation | Natural Products Sciences, vol. 15, no. 3, pp. 144-150 | - |
dc.subject.keyword | 3,5-dimethoxyphenanthrene-2,7-diol | - |
dc.subject.keyword | batatasin-I | - |
dc.subject.keyword | Dioscorea opposita | - |
dc.subject.keyword | Gradient centrifugal partition chromatography (GCPC) | - |
dc.subject.keyword | n-hexane-methanol-water system (HMW, 10 : 9 : 1, v/v) | - |
dc.subject.local | 3,5-dimethoxyphenanthrene-2,7-diol | - |
dc.subject.local | batatasin-I | - |
dc.subject.local | Dioscorea opposita | - |
dc.subject.local | Gradient centrifugal partition chromatography (GCPC) | - |
dc.subject.local | n-hexane-methanol-water system (HMW, 10 : 9 : 1, v/v) | - |
dc.description.journalClass | N | - |
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