Enhancement of recombinant antibody production in HEK 293E cells by WPRE

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dc.contributor.authorK S Kim-
dc.contributor.authorM S Kim-
dc.contributor.authorJihyun Moon-
dc.contributor.authorMun Sik Jeong-
dc.contributor.authorJinhong Kim-
dc.contributor.authorG M Lee-
dc.contributor.authorP K Myung-
dc.contributor.authorHyo Jeong Hong-
dc.date.accessioned2017-04-19T09:16:14Z-
dc.date.available2017-04-19T09:16:14Z-
dc.date.issued2009-
dc.identifier.issn1226-8372-
dc.identifier.uri10.1007/s12257-008-0221-2ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9282-
dc.description.abstractIn an effort to make a fast and convenient approach for efficient production of recombinant antibody, transient gene expression was performed in human embryonic kidney 293E (HEK293E) cells, which have been widely used as a mammalian host for transient expression of recombinant proteins. Woodchuck hepatitis virus post-transcriptional regulation element (WPRE) was employed to increase the antibody production. Under the influence of WPRE, the antibody production was increased by 5.5-fold through the enhancement of total mRNA levels of HC and LC, and the efficient export of nuclear mRNA into the cytoplasm. Using WPRE, 1.9 mg of cumulative recombinant antibody was obtained in transiently transfected adherent HEK293E cells from one 100 mm dish transfection with 10 mL medium exchange every 3 days for 24 days of cultivation. In addition, the highest recombinant antibody concentration of 81 mg/L was obtained. This simple and efficient approach of antibody production is expected to provide a sufficient amount of antibody for screening experiments.-
dc.publisherSpringer-
dc.titleEnhancement of recombinant antibody production in HEK 293E cells by WPRE-
dc.title.alternativeEnhancement of recombinant antibody production in HEK 293E cells by WPRE-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number5-
dc.citation.endPage638-
dc.citation.startPage633-
dc.citation.volume14-
dc.contributor.affiliatedAuthorJihyun Moon-
dc.contributor.affiliatedAuthorMun Sik Jeong-
dc.contributor.affiliatedAuthorJinhong Kim-
dc.contributor.affiliatedAuthorHyo Jeong Hong-
dc.contributor.alternativeName김근수-
dc.contributor.alternativeName김민수-
dc.contributor.alternativeName문지현-
dc.contributor.alternativeName정문식-
dc.contributor.alternativeName김진홍-
dc.contributor.alternativeName이균민-
dc.contributor.alternativeName명평근-
dc.contributor.alternativeName홍효정-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 14, no. 5, pp. 633-638-
dc.identifier.doi10.1007/s12257-008-0221-2-
dc.subject.keywordHEK 293E cells-
dc.subject.keywordMRNA export-
dc.subject.keywordMRNA stability-
dc.subject.keywordRecombinant antibody-
dc.subject.keywordTransient expression-
dc.subject.keywordWPRE-
dc.subject.localHEK 293E cells-
dc.subject.localMRNA export-
dc.subject.localMRNA stability-
dc.subject.localmRNA stability-
dc.subject.localRecombinant antibody-
dc.subject.localrecombinant antibody-
dc.subject.localtransient expression-
dc.subject.localTransient expression-
dc.subject.localWPRE-
dc.description.journalClassY-
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