Production of pure β-glucan by Aureobasidium pullulans after pullulan synthetase gene disruption = pul 유전자가 제거된 Aureobasidium pullulan에 의한 순수 β-glucan 생산

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Title
Production of pure β-glucan by Aureobasidium pullulans after pullulan synthetase gene disruption = pul 유전자가 제거된 Aureobasidium pullulan에 의한 순수 β-glucan 생산
Author(s)
Byeong Gwan Kang; Heejong Yang; Nack-Shick Choi; Keug Hyun AhnChan Sun Park; Byung Dae Yoon; Min-Soo Kim
Bibliographic Citation
Biotechnology Letters, vol. 32, no. 1, pp. 137-142
Publication Year
2010
Abstract
By disruption of the pullulan synthetase gene (pul) of Aureobasidium pullulans IMS822 KCTC11179BP, we constructed a mutant strain, A. pullulans NP1221, which produced a pure β-glucan exopolysaccharide. The mutant NP1221 was white, whereas the wild-type strain produced a black dye. When we compared fermentation kinetics between wide-type and mutant strains, the mutant NP1221 did not produce pullulan. Substrate uptake rate and β-glucan production were similar in both strains. However, the biomass yield of mutant NP1221 was 2.3-fold (9.2 g l-1) greater than that of wild-type.
Keyword
β-GlucanAureobasidium pullulansHomologous recombinationPullulan
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-009-0127-x
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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