Production of pure β-glucan by Aureobasidium pullulans after pullulan synthetase gene disruption = pul 유전자가 제거된 Aureobasidium pullulan에 의한 순수 β-glucan 생산

Abstract

By disruption of the pullulan synthetase gene (pul) of Aureobasidium pullulans IMS822 KCTC11179BP, we constructed a mutant strain, A. pullulans NP1221, which produced a pure β-glucan exopolysaccharide. The mutant NP1221 was white, whereas the wild-type strain produced a black dye. When we compared fermentation kinetics between wide-type and mutant strains, the mutant NP1221 did not produce pullulan. Substrate uptake rate and β-glucan production were similar in both strains. However, the biomass yield of mutant NP1221 was 2.3-fold (9.2 g l-1) greater than that of wild-type.