The quorum sensing-dependent gene katG of Burkholderia glumae is important for protection from visible light

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Title
The quorum sensing-dependent gene katG of Burkholderia glumae is important for protection from visible light
Author(s)
H Chun; O Choi; E Goo; N Kim; H Kim; Y Kang; J Kim; Jae Sun Moon; I Hwang
Bibliographic Citation
Journal of Bacteriology, vol. 191, no. 13, pp. 4152-4157
Publication Year
2009
Abstract
Quorum sensing (QS) plays important roles in the pathogenicity of Burkholderia glumae, the causative agent of bacterial rice grain rot. We determined how QS is involved in catalase expression in B. glumae. The QS-defective mutant of B. glumae exhibited less catalase activity than wild-type B. glumae. A β-glucuronidase assay of a katG::Tn3-gusA78 reporter fusion protein revealed that katG expression is under the control of QS. Furthermore, katG expression was upregulated by QsmR, a transcriptional activator for flagellar-gene expression that is regulated by QS. A gel mobility shift assay confirmed that QsmR directly activates katG expression. The katG mutant produced toxoflavin but exhibited less severe disease than BGR1 on rice panicles. Under visible light conditions and a photon flux density of 61.6 μmol-1 m-2, the survival rate of the katG mutant was 105-fold lower than that of BGR1. This suggests that KatG is a major catalase that protects bacterial cells from visible light, which probably results in less severe disease caused by the katG mutant.
ISSN
0021-9193
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/JB.00227-09
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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