Activity assay for nisin-like acidic bacteriocins using an optimal pH-conditioned gel matrix = pH 조절 gel matrix를 이용한 nisin 유사 baceriocin의 활성 분석

Cited 2 time in scopus
Metadata Downloads
Title
Activity assay for nisin-like acidic bacteriocins using an optimal pH-conditioned gel matrix = pH 조절 gel matrix를 이용한 nisin 유사 baceriocin의 활성 분석
Author(s)
Nack-Shick Choi; Sung Yeop Jung; Heejong Yang; Keug Hyun AhnChan Sun ParkCha Young KimJoong Su Kim; Byung Dae Yoon; Min-Soo Kim
Bibliographic Citation
Analytical Biochemistry, vol. 397, no. 2, pp. 259-261
Publication Year
2010
Abstract
A new zymography for detecting nisin-like acidic bacteriocins was developed using a tricine-sodium dodecyl sulfate (SDS) gel and an acidic gel matrix (pH 4.0). After electrophoresis, proteins in the tricine gel were electrotransferred to an optimal pH-conditioned gel matrix (OP-CGM). The OP-CGM was overlaid with indicator cells (Bacillus cereus) embedded in nutrient broth soft agar (0.8%, w/v). Antibacterial activity shown as a growth inhibition using B. cereus was detected at approximately 3.8 kDa. Because nisin is unstable in buffers at pH values over 6.0, the common electrophoretic systems, SDS-polyacrylamide gel electrophoresis and tricine gel, are not suitable for detection of nisin-like acidic bacteriocins.
ISSN
0003-2697
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.ab.2009.09.046
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
Jeonbuk Branch Institute > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.