DC Field | Value | Language |
---|---|---|
dc.contributor.author | H J Kim | - |
dc.contributor.author | B C Lim | - |
dc.contributor.author | Soo Jin Yeom | - |
dc.contributor.author | Y S Kim | - |
dc.contributor.author | Doo Il Kim | - |
dc.contributor.author | D K Oh | - |
dc.date.accessioned | 2017-04-19T09:16:48Z | - |
dc.date.available | 2017-04-19T09:16:48Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | 10.1007/s10529-009-0115-1 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9315 | - |
dc.description.abstract | Using site-directed mutagenesis, we investigated the roles of Ile66 and Ala107 of d-psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its true substrate, D-fructose. When Ile66 was substituted with alanine, glycine, cysteine, leucine, phenylalanine, tryptophan, tyrosine or valine, all the mutants dramatically increased the Km for d-tagatose but slightly decreased the Km for d-fructose, indicating that Ile66 is involved in substrate recognition. When Ala107 was substituted by either isoleucine or valine, the substituted mutants had lower thermostability than the wild-type enzyme whereas the proline-substituted mutant had higher thermostability. Thus, Ala107 is involved in enzyme stability. | - |
dc.publisher | Springer | - |
dc.title | Roles of Ile66 and Ala107 of d-psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its substrate, d-fructose | - |
dc.title.alternative | Roles of Ile66 and Ala107 of d-psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its substrate, d-fructose | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Letters | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 118 | - |
dc.citation.startPage | 113 | - |
dc.citation.volume | 32 | - |
dc.contributor.affiliatedAuthor | Soo Jin Yeom | - |
dc.contributor.affiliatedAuthor | Doo Il Kim | - |
dc.contributor.alternativeName | 김혜정 | - |
dc.contributor.alternativeName | 임병철 | - |
dc.contributor.alternativeName | 염수진 | - |
dc.contributor.alternativeName | 김영수 | - |
dc.contributor.alternativeName | 김두일 | - |
dc.contributor.alternativeName | 오덕근 | - |
dc.identifier.bibliographicCitation | Biotechnology Letters, vol. 32, no. 1, pp. 113-118 | - |
dc.identifier.doi | 10.1007/s10529-009-0115-1 | - |
dc.subject.keyword | Agrobacterium tumefaciens | - |
dc.subject.keyword | D-Psicose 3-epimerase | - |
dc.subject.keyword | Protein stability | - |
dc.subject.keyword | Site-directed mutagenesis | - |
dc.subject.keyword | Substrate specificity | - |
dc.subject.local | agrobacterium tumefaciens | - |
dc.subject.local | Agrobacterium tumefaciens | - |
dc.subject.local | d-Psicose 3-epimerase | - |
dc.subject.local | D-Psicose 3-epimerase | - |
dc.subject.local | Protein stability | - |
dc.subject.local | protein stability | - |
dc.subject.local | site-directed mutagenesis | - |
dc.subject.local | Site-directed mutagenesis | - |
dc.subject.local | Substrate specificity | - |
dc.subject.local | substrate specificity | - |
dc.description.journalClass | Y | - |
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