DC Field | Value | Language |
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dc.contributor.author | Lan Hee Lee | - |
dc.contributor.author | Kim Do Young | - |
dc.contributor.author | M K Han | - |
dc.contributor.author | Hyun Woo Oh | - |
dc.contributor.author | Su Jin Ham | - |
dc.contributor.author | Doo Sang Park | - |
dc.contributor.author | Kyung Sook Bae | - |
dc.contributor.author | D E Sok | - |
dc.contributor.author | D H Shin | - |
dc.contributor.author | Kwang Hee Son | - |
dc.contributor.author | Ho Yong Park | - |
dc.date.accessioned | 2017-04-19T09:16:54Z | - |
dc.date.available | 2017-04-19T09:16:54Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0440-2413 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9323 | - |
dc.description.abstract | A xylan-decomposing bacterium, HY-20, was isolated from the gut of a honeybee, Apis mellifera, and identified as Bacillus sp. The extracellular GH11 xylanase (XylP) gene (687-bp) of strain HY-20 encoded a protein of 228 amino acids with a deduced molecular mass of 25,522 Da and a calculated pI of 9.33. The primary structure of XylP was 97% identical to that of B. pumilus xylanase (GenBank accession no.: AY526092) that has not been characterized yet. The recombinant His-tagged enzyme (rXylP) overexpressed in Escherichia coli BL21 harboring pET-28a(+)/xylP was purified to electrophoretic homogeneity by cation exchange and gel permeation chromatographies. The purified enzyme exhibited the highest catalytic activity toward birchwood xylan at pH 6.5 and 50°C and retained approximately 50% of its original activity when pre-incubated at 55°C for 15 min. The recombinant enzyme was completely inactivated by Hg2+ (1 mM) and N-bromosuccinimide (5 mM), while its activity was slightly stimulated by approximately 10% in the presence of Mn2+ (1 mM), Fe2+ (1 mM), and sodium azide (5 mM). rXylP was able to efficiently degrade various polymeric xylose-based substrates but PNP-sugar derivatives and glucose-based polymers were not susceptible to the enzyme. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Characterization of an extracellular xylanase from Bacillus sp. HY-20, a bacterium in the gut of Apis mellifera = 꿀벌(Apis mellifera)의 장내 세균인 Bacillus sp. HY-20이 분비하는 Xylanase의 특성 | - |
dc.title.alternative | Characterization of an extracellular xylanase from Bacillus sp. HY-20, a bacterium in the gut of Apis mellifera | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of Microbiology | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 338 | - |
dc.citation.startPage | 332 | - |
dc.citation.volume | 45 | - |
dc.contributor.affiliatedAuthor | Lan Hee Lee | - |
dc.contributor.affiliatedAuthor | Kim Do Young | - |
dc.contributor.affiliatedAuthor | Hyun Woo Oh | - |
dc.contributor.affiliatedAuthor | Su Jin Ham | - |
dc.contributor.affiliatedAuthor | Doo Sang Park | - |
dc.contributor.affiliatedAuthor | Kyung Sook Bae | - |
dc.contributor.affiliatedAuthor | Kwang Hee Son | - |
dc.contributor.affiliatedAuthor | Ho Yong Park | - |
dc.contributor.alternativeName | 이란희 | - |
dc.contributor.alternativeName | 김도영 | - |
dc.contributor.alternativeName | 한미경 | - |
dc.contributor.alternativeName | 오현우 | - |
dc.contributor.alternativeName | 함수진 | - |
dc.contributor.alternativeName | 박두상 | - |
dc.contributor.alternativeName | 배경숙 | - |
dc.contributor.alternativeName | 석대은 | - |
dc.contributor.alternativeName | 신동하 | - |
dc.contributor.alternativeName | 손광희 | - |
dc.contributor.alternativeName | 박호용 | - |
dc.identifier.bibliographicCitation | Korean Journal of Microbiology, vol. 45, no. 4, pp. 332-338 | - |
dc.subject.keyword | Apis mellifera | - |
dc.subject.keyword | Bacillus sp. HY-20 | - |
dc.subject.keyword | Bee | - |
dc.subject.keyword | Gut bacterium | - |
dc.subject.keyword | Xylanase | - |
dc.subject.local | Apis mellifera | - |
dc.subject.local | Bacillus sp. HY-20 | - |
dc.subject.local | Bee | - |
dc.subject.local | Gut bacterium | - |
dc.subject.local | Xylanase | - |
dc.subject.local | xylanase | - |
dc.description.journalClass | N | - |
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