Iloprost, a prostacyclin analogue, stimulates meiotic maturation and early embryonic development in pigs = 돼지 체외 성숙 및 초기 배 발달에서 Iloprost의 역할

Cited 20 time in scopus
Metadata Downloads
Title
Iloprost, a prostacyclin analogue, stimulates meiotic maturation and early embryonic development in pigs = 돼지 체외 성숙 및 초기 배 발달에서 Iloprost의 역할
Author(s)
Ji Su Kim; J I Chae; Bong Seok SongKyu-Sun Lee; Y K Choo; Kyu Tae Chang; H Park; Deog Bon Koo
Bibliographic Citation
Reproduction Fertility and Development, vol. 22, no. 2, pp. 437-447
Publication Year
2010
Abstract
Oviduct fluid contains various cytokines and growth factors that enhance the embryo development during the preimplantation period. In hatched embryos, prostacyclin (PGI2) improves implantation, but its role during oocyte maturation and early embryo development remains contentious. Therefore, in the present study, we examined the effects of a PGI2 analogue (iloprost) on meiotic maturation and early embryonic development in pigs, as well on the structural integrity, mitochondrial membrane potential and apoptosis in blastocysts. First, meiotic maturation in pig oocytes was examined in the presence of increasing concentrations of iloprost (1, 5 and 10 μM). After IVM, a higher proportion of iloprost-treated compared with untreated oocytes was in MII (90.0% v. 65.7%, respectively; P < 0.05). In addition, protein kinase A activity increased in iloprost-treated oocytes, indicating increased intracellular cAMP concentrations. After 22 h iloprost treatment (44 h total incubation time), western blotting demonstrated increased expression of extracellular signal-regulated kinase (ERK) 1/2, phosphorylated (p-) ERK1/2, cAMP response element-binding protein (CREB), p-CREB and cyclo-oxygenase-2, indicating activation of the mitogen-activated protein kinase and PGI 2 pathways. In addition, the frequency of polyspermy decreased in iloprost-treated oocytes (19.9%) compared with control (35.8%), whereas the rate of blastocyst formation increased (P < 0.05). Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) showed that the number of nuclei containing fragmented DNA at the blastocyst stage decreased in the iloprost-treated group compared with control (1.2% v. 3.6%, respectively). In conclusion, iloprost appears to play a direct role in porcine oocyte maturation by enhancing blastocyst structure and survival.
Keyword
Blastocyst development
ISSN
1031-3613
Publisher
CSIRO Publishing
DOI
http://dx.doi.org/10.1071/RD08287
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.