Proteolytic fluorescent signal amplification on gold nanoparticles for a highly sensitive and rapid protease assay

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Title
Proteolytic fluorescent signal amplification on gold nanoparticles for a highly sensitive and rapid protease assay
Author(s)
Kim Joong Hyun; Bong Hyun Chung
Bibliographic Citation
Small, vol. 6, no. 1, pp. 126-131
Publication Year
2010
Abstract
A new strategy for highly sensitive and rapid protease assay is developed by mediating proteolytic formation of oligonucleotide duplexes and using the duplexes for signal amplification. In the presence of matrix metalloprotease2 (MMP-2), fragmentation of the intact DNA-peptide on gold nanoparticles (GNP) by hydrolytic cleavage of a peptide bond within the substrate allows diffusion of DNA away from the GNP and the formation of a DNA/RNA heteroduplex, leading to digestion of RNA by RNase H. Because of the high quenching efficacy of GNP to the fluorophore in RNA and multiple digestions of the RNA, the fluorescence signal recovery is amplified. This method permits the assessment of the activity of MMP-2 at concentrations as low as 10 pM within 4h. Compared with the reported protease nanosensors using quantum dots, GNP, and magnetic nanoparticles with the same peptide sequence, the assay time of this method is sixfold faster and the limit of detection is 100-fold more sensitive. The formulations for proteolytic formations of oligonucleotides duplexes for signal amplification on GNP could lead to the development of more sensitive and rapid protease assay techniques, thus extending the role of proteases as therapeutic targets and disease indicators.
Keyword
FRETGold nanoparticlesOligonucleotidesPeptidesProtease assay
ISSN
1613-6810
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/smll.200901635
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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