Metabolic profiles of genetically modified potatoes using a combination of metabolite fingerprinting and multivariate analysis

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dc.contributor.authorHyun Soon Kim-
dc.contributor.authorSuk Weon Kim-
dc.contributor.authorY S Park-
dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorJang Ryol Liu-
dc.contributor.authorHyouk Joung-
dc.contributor.authorJae Heung Jeon-
dc.date.accessioned2017-04-19T09:17:15Z-
dc.date.available2017-04-19T09:17:15Z-
dc.date.issued2009-
dc.identifier.issn12268372-
dc.identifier.uri10.1007/s12257-009-0168-yko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9358-
dc.description.abstractComprehensive metabolite fingerprinting of transgenic potatoes that constitutively express human beta amyloid, curdlan synthase (CRDS), and glycogen synthase (glgA); and of wild-type potatoes was carried out using FT-IR and 1H NMR spectroscopy in combination with multivariate analyses. Comparison of metabolic patterns between transgenic and wild-type potatoes revealed that there were neither quantitative nor qualitative differences in metabolites between transgenic potatoes expressing human beta amyloid, CRDS or glgA, and non-transformed control potatoes. However, there were metabolic differences between two control potato lines - one that was fresh and the other stored. After 1 week of storage, comprehensive metabolite patterns were significantly modified. Although the differences between CRDS and glgA transgenic and control potato lines were small, PCA analysis of FT-IR and 1H NMR spectral data identified two distinct control lines. These results suggest that the comprehensive metabolite changes in control potato lines, which occurred after 1 week of storage, were greater than the differences between CRDS and glgA transgenic and wild-type potato lines. Thus, the combination of FT-IR and 1H NMR spectral data and multivariate analysis was valuable for the detection of comprehensive differences in metabolic profiles between transgenic and non-transformed control plants, even though peak-signal overlap prevented assignment of pure compounds. The combination of FT-IR and 1H NMR spectral data and multivariate analysis is a simple and rapid method for evaluation of the metabolic equivalence of GM crops.-
dc.publisherSouth Korea-
dc.titleMetabolic profiles of genetically modified potatoes using a combination of metabolite fingerprinting and multivariate analysis-
dc.title.alternativeMetabolic profiles of genetically modified potatoes using a combination of metabolite fingerprinting and multivariate analysis-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number6-
dc.citation.endPage747-
dc.citation.startPage738-
dc.citation.volume14-
dc.contributor.affiliatedAuthorHyun Soon Kim-
dc.contributor.affiliatedAuthorSuk Weon Kim-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.affiliatedAuthorJae Heung Jeon-
dc.contributor.alternativeName김현순-
dc.contributor.alternativeName김석원-
dc.contributor.alternativeName박영석-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName유장렬-
dc.contributor.alternativeName정혁-
dc.contributor.alternativeName전재흥-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 14, no. 6, pp. 738-747-
dc.identifier.doi10.1007/s12257-009-0168-y-
dc.subject.keyword1H NMR spectroscopy-
dc.subject.keywordFT-IR spectroscopy-
dc.subject.keywordGenetically modified potatoes-
dc.subject.keywordMultivariate analysis-
dc.subject.keywordPrincipal component analysis (PCA)-
dc.subject.local1H NMR spectroscopy-
dc.subject.localFT-IR spectroscopy-
dc.subject.localFourier transform infrared spectroscopy (FT-IR)-
dc.subject.localGenetically modified potatoes-
dc.subject.localMultivariate analysis-
dc.subject.localPrincipal component analysis (PCA)-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Biomaterials Research > 1. Journal Articles
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