Application of rpoB and Zinc Protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies

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dc.contributor.authorH S Kim-
dc.contributor.authorDae Sil Lee-
dc.contributor.authorYoung Hyo Chang-
dc.contributor.authorM J Kim-
dc.contributor.authorS Koh-
dc.contributor.authorJoong Su Kim-
dc.contributor.authorJ H Seong-
dc.contributor.authorS K Song-
dc.contributor.authorH S Shin-
dc.contributor.authorJ B Son-
dc.contributor.authorMin-Young Jung-
dc.contributor.authorS N Park-
dc.contributor.authorS Y Yoo-
dc.contributor.authorK W Cho-
dc.contributor.authorD K Kim-
dc.contributor.authorSeong Hoon Moon-
dc.contributor.authorD Kim-
dc.contributor.authorY Choi-
dc.contributor.authorB O Kim-
dc.contributor.authorH S Jang-
dc.contributor.authorC S Kim-
dc.contributor.authorC Kim-
dc.contributor.authorS J Choe-
dc.contributor.authorJ K Kook-
dc.date.accessioned2017-04-19T09:17:19Z-
dc.date.available2017-04-19T09:17:19Z-
dc.date.issued2010-
dc.identifier.issn0095-1137-
dc.identifier.uri10.1128/JCM.01631-09ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9386-
dc.description.abstractFusobacterium nucleatum is classified into five subspecies that inhabit the human oral cavity (F. nucleatum subsp. nucleatum, F. nucleatum subsp. polymorphum, F. nucleatum subsp. fusiforme, F. nucleatum subsp. vincentii, and F. nucleatum subsp. animalis) based on several phenotypic characteristics and DNA-DNA hybridization patterns. However, the methods for detecting or discriminating the clinical isolates of F. nucleatum at the subspecies levels are laborious, expensive, and time-consuming. Therefore, in this study, the nucleotide sequences of the RNA polymerase β-subunit gene (rpoB) and zinc protease gene were analyzed to discriminate the subspecies of F. nucleatum. The partial sequences of rpoB (approximately 2,419 bp), the zinc protease gene (878 bp), and 16S rRNA genes (approximately 1,500 bp) of the type strains of five subspecies, 28 clinical isolates of F. nucleatum, and 10 strains of F. periodonticum (as a control group) were determined and analyzed. The phylogenetic data showed that the rpoB and zinc protease gene sequences clearly delineated the subspecies of F. nucleatum and provided higher resolution than the 16S rRNA gene sequences in this respect. According to the phylogenetic analysis of rpoB and the zinc protease gene, F. nucleatum subsp. vincentii and F. nucleatum subsp. fusiforme might be classified into a single subspecies. Five clinical isolates could be delineated as a new subspecies of F. nucleatum. The results suggest that rpoB and the zinc protease gene are efficient targets for the discrimination and taxonomic analysis of the subspecies of F. nucleatum.-
dc.publisherAmer Soc Microb-
dc.titleApplication of rpoB and Zinc Protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies-
dc.title.alternativeApplication of rpoB and Zinc Protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies-
dc.typeArticle-
dc.citation.titleJournal of Clinical Microbiology-
dc.citation.number2-
dc.citation.endPage553-
dc.citation.startPage545-
dc.citation.volume48-
dc.contributor.affiliatedAuthorDae Sil Lee-
dc.contributor.affiliatedAuthorYoung Hyo Chang-
dc.contributor.affiliatedAuthorJoong Su Kim-
dc.contributor.affiliatedAuthorMin-Young Jung-
dc.contributor.affiliatedAuthorSeong Hoon Moon-
dc.contributor.alternativeName김화숙-
dc.contributor.alternativeName이대실-
dc.contributor.alternativeName장영효-
dc.contributor.alternativeName김민정-
dc.contributor.alternativeName고석훈-
dc.contributor.alternativeName김중수-
dc.contributor.alternativeName성진효-
dc.contributor.alternativeName송수근-
dc.contributor.alternativeName신환선-
dc.contributor.alternativeName손재범-
dc.contributor.alternativeName정민영-
dc.contributor.alternativeName박순낭-
dc.contributor.alternativeName유소영-
dc.contributor.alternativeName조기운-
dc.contributor.alternativeName김동기-
dc.contributor.alternativeName문성훈-
dc.contributor.alternativeName김두일-
dc.contributor.alternativeName최용석-
dc.contributor.alternativeName김병옥-
dc.contributor.alternativeName장현선-
dc.contributor.alternativeName김춘성-
dc.contributor.alternativeName김찬-
dc.contributor.alternativeName채선진-
dc.contributor.alternativeName국중기-
dc.identifier.bibliographicCitationJournal of Clinical Microbiology, vol. 48, no. 2, pp. 545-553-
dc.identifier.doi10.1128/JCM.01631-09-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Bio-Infrastructure Policy Support Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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