Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100

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Title
Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100
Author(s)
Kwon HwangBo; Su Hyun Son; Jong Suk Lee; Sung Ran Min; S M Ko; Jang Ryol Liu; D Choi; Won Joong Chung
Bibliographic Citation
Plant Biotechnology Reports, vol. 4, no. 1, pp. 49-52
Publication Year
2010
Abstract
A DNA extraction method using Chelex 100 is widely used for bacteria, Chlamydomonas, and animal cell lines, but only rarely for plant materials due to the need for additional time-consuming and tedious steps. We have modified the Chelex 100 protocol and successfully developed a rapid and simple method of DNA extraction for efficient PCR-based detection of transgenes from a variety of transgenic plant and algal species. Our protocol consists of homogenizing plant tissue with a pestle, boiling the homogenized tissue in a microfuge tube with 5% Chelex 100 for 5 min, and centrifuging the boiled mixture. The supernatant, which is used for PCR analysis, was able to successfully amplify transgenes in transgenic tobacco, tomato, potato, Arabidopsis, rice, strawberry, Spirodela polyrhiza, Chlamydomonas, and Porphyra tenera. The entire DNA extraction procedure requires <15 min and is therefore comparable to that used for bacteria, Chlamydomonas, and animal cell lines.
Keyword
Chelex 100DNA extraction methodPCRPlant materialTransgenes
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-009-0117-4
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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