Development of one-step real-time reverse transcription polymerase chain reaction assays for rapid detection of porcine group C rotaviruses

Cited 8 time in scopus
Metadata Downloads
Title
Development of one-step real-time reverse transcription polymerase chain reaction assays for rapid detection of porcine group C rotaviruses
Author(s)
Y H Chun; Y J Jeong; S I Park; M Hosmillo; D J Shin; H J Kwon; S Y Kang; S K Woo; M I Kang; Su-Jin Park; K O Cho
Bibliographic Citation
Journal of Veterinary Diagnostic Investigation, vol. 22, no. 1, pp. 74-77
Publication Year
2010
Abstract
Although the widespread occurrence of porcine group C rotaviruses (GCRV) is assumed, precise prevalence remains largely unknown because of the absence of reliable, specific, and rapid diagnostic methods. To detect and quantify porcine GCRV, the authors evaluated and optimized SYBR Green and TaqMan real-time reverse transcription polymerase chain reaction (RT-PCR) assays and applied them to 108 piglet fecal samples. Using serially diluted standard RNA transcripts of porcine GCRV VP6 gene, both SYBR Green and TaqMan real-time RT-PCR assays detected as few as 1 3 101 genome copies/ml (correlation coefficiency .0.99), whereas conventional RT-PCR detected 1.0 3 103 copies/ml. In addition, the conventional assay detected porcine GCRV in 24% (26/108) of fecal samples, whereas the detection rates of both SYBR Green and TaqMan assays were 72% (78 of 108) and 64% (70 of 108), respectively. The current study indicated that both real-time RT-PCR assays were reliable, specific, and rapid methods for the detection of porcine GCRV in porcine fecal samples.
Keyword
DiagnosisPorcine group c rotavirusReal-time reverse transcription polymerase chain reactionSybr greenTaqman
ISSN
1040-6387
Publisher
Sage
DOI
http://dx.doi.org/10.1177/104063871002200113
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.