Secretory production of recombinant proteins in Escherichia coli

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Title
Secretory production of recombinant proteins in Escherichia coli
Author(s)
Sung Ho Yoon; Seong Keun Kim; Jihyun Kim
Bibliographic Citation
Recent Patents on Biotechnology, vol. 4, no. 1, pp. 23-29
Publication Year
2010
Abstract
Extracellular production of heterologous proteins using the Escherichia coli cell factory offers several advantages over intracellular production and mammalian culture. Properly folded proteins can be rapidly accumulated in the culture media, and downstream processes for isolation and purification can be much simplified. Efforts to enhance the secretory production of target proteins can be largely classified as selection and modification of the signal peptide, co-expression of proteins to assist translocation and folding, improvement of periplasmic release, and protection of target proteins from degradation and contamination. Here, we review recent patents on the secretory production of recombinant proteins in E. coli.
Keyword
Secretionexcretionperiplasmextracellular productionsignal peptiderecombinant proteinEscherichia coli.
ISSN
1872-2083
Publisher
Bentham Science Publishers
DOI
http://dx.doi.org/10.2174/187220810790069550
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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