DC Field | Value | Language |
---|---|---|
dc.contributor.author | C Hwangbo | - |
dc.contributor.author | J Kim | - |
dc.contributor.author | Jung Joon Lee | - |
dc.contributor.author | J H Lee | - |
dc.date.accessioned | 2017-04-19T09:17:29Z | - |
dc.date.available | 2017-04-19T09:17:29Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.uri | 10.1158/0008-5472.CAN-09-2447 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9428 | - |
dc.description.abstract | Aberrant adhesion signaling pathways in cancer cells underlie their deadly invasive capabilities. The adhesion-related PDZ adapter protein mda-9/syntenin is a positive regulator of cancer cell progression in breast cancer, melanoma, and other human cancers. In this study, we report that mda-9/syntenin mediates adhesion-mediated activation of protein kinase Cα (PKCα) and focal adhesion kinase (FAK) by fibronectin (FN) in human breast cancer and melanoma cells. FN rapidly stimulated the expression of mda-9/syntenin and the activation of PKCα prior to activation of FAK. Inhibiting PKCα suppressed basal or FN-induced expression of mda-9/syntenin, as well as cell migration and invasion toward FN stimulated by mda-9/syntenin. Several lines of evidence suggested that activation of PKCα and expression of mda-9/syntenin were interdependent. First, mda-9/syntenin inhibition suppressed basal or FN-induced phosphorylation of PKCα at Thr638/641, whereas PKCα inhibition suppressed basal or FN-induced expression of mda-9/syntenin. Second, inhibiting either mda-9/syntenin or PKCα suppressed FN-induced formation of integrin-β1/FAK/c-Src signaling complexes. Third, inhibiting either mda-9/syntenin or PKCα suppressed FN-induced phosphorylation of FAK Tyr397 and c-Src Tyr416 and the induction of downstream effector signals to p38 and mitogen-activated protein kinase, Cdc42, and NF-κB. In summary, our findings offer evidence that mda-9/syntenin acts as a molecular adaptor linking PKCα and FAK activation in a pathway of FN adhesion by human breast cancer and melanoma cells. | - |
dc.publisher | Amer Assoc Cancer Research | - |
dc.title | Activation of the integrin effector kinase focal adhesion kinase in cancer cells is regulated by crosstalk between protein kinase Cα and the PDZ adapter protein mda-9/syntenin | - |
dc.title.alternative | Activation of the integrin effector kinase focal adhesion kinase in cancer cells is regulated by crosstalk between protein kinase Cα and the PDZ adapter protein mda-9/syntenin | - |
dc.type | Article | - |
dc.citation.title | Cancer Research | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 1655 | - |
dc.citation.startPage | 1645 | - |
dc.citation.volume | 70 | - |
dc.contributor.affiliatedAuthor | Jung Joon Lee | - |
dc.contributor.alternativeName | 황보철 | - |
dc.contributor.alternativeName | 김재경 | - |
dc.contributor.alternativeName | 이정준 | - |
dc.contributor.alternativeName | 이정형 | - |
dc.identifier.bibliographicCitation | Cancer Research, vol. 70, no. 4, pp. 1645-1655 | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-09-2447 | - |
dc.description.journalClass | Y | - |
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