Stc1 : a critical link between RNAi and chromatin modification required for heterochromatin integrity

Cited 155 time in scopus
Metadata Downloads
Title
Stc1 : a critical link between RNAi and chromatin modification required for heterochromatin integrity
Author(s)
E H Bayne; S A White; A Kagansky; D A Bijos; L Sanchez-Pulido; Kwang Lae Hoe; Dong Uk Kim; H O Park; C P Ponting; J Rappsilber; R C Allshire
Bibliographic Citation
Cell, vol. 140, no. 5, pp. 666-677
Publication Year
2010
Abstract
In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads to recruitment of the CLRC complex, including the histone methyltransferase Clr4, promoting H3K9 methylation and heterochromatin formation. A key question is what mediates the recruitment of Clr4/CLRC to transcript-bound RITS. We have identified a LIM domain protein, Stc1, that is required for centromeric heterochromatin integrity. Our analyses show that Stc1 is specifically required to establish H3K9 methylation via RNAi, and interacts both with the RNAi effector Ago1, and with the chromatin-modifying CLRC complex. Moreover, tethering Stc1 to a euchromatic locus is sufficient to induce silencing and heterochromatin formation independently of RNAi. We conclude that Stc1 associates with RITS on centromeric transcripts and recruits CLRC, thereby coupling RNAi to chromatin modification.
Keyword
CELLBIOSIGNALNIG
ISSN
0092-8674
Publisher
Elsevier-Cell Press
DOI
http://dx.doi.org/10.1016/j.cell.2010.01.038
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.