Shiga toxin A subunit mutant of Escherichia coli O157:H7 releases outer membrane vesicles containing the B-pentameric complex

Cited 11 time in scopus
Metadata Downloads
Title
Shiga toxin A subunit mutant of Escherichia coli O157:H7 releases outer membrane vesicles containing the B-pentameric complex
Author(s)
Sang-Hyun Kim; Sang Rae Lee; K S Kim; A Ko; Ekyune Kim; Y H Kim; Kyu Tae Chang
Bibliographic Citation
FEMS Immunology and Medical Microbiology, vol. 58, no. 3, pp. 412-420
Publication Year
2010
Abstract
Shiga toxins (STx) are secreted extracellularly through the outer membrane vesicles (OMVs) of Escherichia coli O157:H7. In an attempt to produce STxA-deficient OMVs from E. coli O157:H7, site-specific deletions of the stx1A and stx2A subunit genes were carried out. The STxA-deficient phenotype of the stx1A/stx2A mutant was confirmed by Vero cell cytotoxicity and VTEC-RPLA((R)) assay. Western blot analyses showed that the B (STxB) subunits were present without coupling to STxA in the OMVs of the STxA-deficient mutant. Furthermore, STxB was located in its homo-pentameric complexes, as revealed by immunoprecipitation and immunoblotting with anti-STxB antibodies. These results suggest that STxB alone can be oligomerized into the B pentamer in the periplasm, and subsequently entrapped into the OMVs. Determination of the median lethal dose concentration for the OMV preparations suggests that the STxA-deficient OMVs containing STxB complex could be safely used as vaccine delivery vehicles.
Keyword
outer membrane vesicle detoxificationmedian lethal doseShiga toxin A mutationEscherichia coli O157vaccine vehicle.
ISSN
0928-8244
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1111/j.1574-695X.2010.00654.x
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.