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- Shiga toxin A subunit mutant of Escherichia coli O157:H7 releases outer membrane vesicles containing the B-pentameric complex
- Sang-Hyun Kim; Sang Rae Lee; K S Kim; A Ko; Ekyune Kim; Y H Kim; Kyu Tae Chang
- Bibliographic Citation
- FEMS Immunology and Medical Microbiology, vol. 58, no. 3, pp. 412-420
- Publication Year
- Shiga toxins (STx) are secreted extracellularly through the outer membrane vesicles (OMVs) of Escherichia coli O157:H7. In an attempt to produce STxA-deficient OMVs from E. coli O157:H7, site-specific deletions of the stx1A and stx2A subunit genes were carried out. The STxA-deficient phenotype of the stx1A/stx2A mutant was confirmed by Vero cell cytotoxicity and VTEC-RPLA((R)) assay. Western blot analyses showed that the B (STxB) subunits were present without coupling to STxA in the OMVs of the STxA-deficient mutant. Furthermore, STxB was located in its homo-pentameric complexes, as revealed by immunoprecipitation and immunoblotting with anti-STxB antibodies. These results suggest that STxB alone can be oligomerized into the B pentamer in the periplasm, and subsequently entrapped into the OMVs. Determination of the median lethal dose concentration for the OMV preparations suggests that the STxA-deficient OMVs containing STxB complex could be safely used as vaccine delivery vehicles.
- outer membrane vesicle detoxification; median lethal dose; Shiga toxin A mutation; Escherichia
coli O157; vaccine vehicle.
- Oxford Univ Press
- Appears in Collections:
- Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
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