Functional characterization of the glxR deletion mutant of Corynebacterium glutamicum ATCC 13032: Involvement of GlxR in acetate metabolism and carbon catabolite repression

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Title
Functional characterization of the glxR deletion mutant of Corynebacterium glutamicum ATCC 13032: Involvement of GlxR in acetate metabolism and carbon catabolite repression
Author(s)
Sun Yang Park; M W Moon; B Subhadra; J K Lee
Bibliographic Citation
FEMS Microbiology Letters, vol. 304, no. 2, pp. 107-115
Publication Year
2010
Abstract
Recently, a cyclic AMP receptor protein homologue, GlxR, was reported to bind to the upstream regions of several genes involved in the regulation of diverse physiological processes in Corynebacterium glutamicum. However, the function of GlxR has not yet been explored in C. glutamicum in vivo using a glxR deletion mutant. Therefore, this study examines the role of GlxR as a repressor in glyoxylate bypass and carbon catabolite repression (CCR) using a deletion mutant. The disruption of glxR resulted in a severe growth defect, but growth was restored by complementation with the glxR and crp genes from C. glutamicum and Streptomyces coelicolor, respectively. The production of isocitrate lyase (ICL) and malate synthase (MS) was significantly increased in the glxR mutant. The specific activities of both enzymes were increased in the glxR mutant, regardless of the carbon source. In accordance, the promoter activities of ICL and MS using lacZ fusion were derepressed in the glxR mutant. In addition, the glxR mutant exhibited derepression of the gluA gene for glutamate uptake in the presence of glucose, thereby relieving CCR by glucose. These results indicate that GlxR plays an important role in CCR as well as in acetate metabolism.
Keyword
Acetate metabolismCarbon catabolite repressionCorynebacterium glutamicumCyclic AMP receptor proteinGlxR
ISSN
0378-1097
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1111/j.1574-6968.2009.01884.x
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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