Preparative isolation and purification of deoxypodophyllotoxin from the rhizomes of Anthriscus sylvestris by high-speed counter-current chromatography = HSCCC를 이용한 전호의 deoxypodophyllotoxin 분리

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Title
Preparative isolation and purification of deoxypodophyllotoxin from the rhizomes of Anthriscus sylvestris by high-speed counter-current chromatography = HSCCC를 이용한 전호의 deoxypodophyllotoxin 분리
Author(s)
G H Quan; Young Won Chin; Hyeong Kyu Lee; Sei Ryang Oh
Bibliographic Citation
Journal of Korean Society for Applied Biological Chemistry, vol. 53, no. 1, pp. 110-113
Publication Year
2010
Abstract
A simple and rapid purification method of deoxypodophyllotoxin from the crude methanol extract of rhizomes of Anthriscus sylvestris was established using high-speed counter-current chromatography. From the crude extract (288.9 mg), deoxypodophyllotoxin (8.8 mg) was separated using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (7:3:5:5, v/v). The final purity of the deoxypodophyllotoxin was determined to be over 98% by ultra-performance liquid chromatography-UV analysis.
Keyword
Anthriscus sylvestrisdeoxypodophyllotoxinhigh-speed counter-current chromatography (HSCCC)
ISSN
I000-0171
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.3839/jksabc.2010.019
Type
Article
Appears in Collections:
Ochang Branch Institute > 1. Journal Articles
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