A novel kanamycin/G418 resistance marker for direct selection of transformants in Escherichia coli and different yeast species = 대장균과 다양한 효모에서 형질전환체를 선별할 수 있는 신규 항생제 저항성 마커

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Title
A novel kanamycin/G418 resistance marker for direct selection of transformants in Escherichia coli and different yeast species = 대장균과 다양한 효모에서 형질전환체를 선별할 수 있는 신규 항생제 저항성 마커
Author(s)
M Agaphonov; N Romanova; Eui Sung Choi; M Ter-Avanesyan
Bibliographic Citation
Yeast, vol. 27, no. 4, pp. 189-195
Publication Year
2010
Abstract
We have developed a set of cloning vectors possessing a modified Tn903 kanamycin resistance gene that enables the selection of both kanamycin-resistant transformants in Escherichia coli and G418-resistant transformants in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha and Pichia pastoris. Expression of this gene in yeast is controlled by the H. polymorpha glyceraldehyde-3-phosphate dehydrogenase promoter, while expression in E. coli is governed by an upstream E. coli lacZ promoter. Applicability of the vectors for gene disruption in H. polymorpha and S. cerevisiae was demonstrated by inactivation of the HpMAL1 and URA3 genes, respectively. One of the vectors possesses a H. polymorpha ARS allowing plasmid maintenance in an episomal state. The small size of the vectors (2-2.5 kb) makes them convenient for routine DNA cloning. In addition, we report a novel approach for construction of gene disruption cassettes.
Keyword
Antibiotic resistanceCloning vectorGene disruptionTransformationYeast
ISSN
0749-503X
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/yea.1741
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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