Obovatol attenuates microglia-mediated neuroinflammation by modulating redox regulation = 오보바톨에 의한 신경 염증 조절 기전규명

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dc.contributor.authorJ Ock-
dc.contributor.authorH S Han-
dc.contributor.authorS H Hong-
dc.contributor.authorS Y Lee-
dc.contributor.authorYoung Min Han-
dc.contributor.authorByoung-Mog Kwon-
dc.contributor.authorK Suk-
dc.date.accessioned2017-04-19T09:18:18Z-
dc.date.available2017-04-19T09:18:18Z-
dc.date.issued2010-
dc.identifier.issn00071188-
dc.identifier.uri10.1111/j.1476-5381.2010.00659.xko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9468-
dc.description.abstractBackground and purpose: Obovatol isolated from the medicinal herb Magnolia obovata exhibits a variety of biological activities. Here, the effect of obovatol and its mechanism of action on microglial activation, neuroinflammation and neurodegeneration were investigated. Experimental approach: In microglial BV-2 cells stimulated with lipopolysaccharide (LPS), we measured nitric oxide (NO) and cytokine production, and activation of intracellular signalling pathways by reverse transcription-polymerase chain reaction and Western blots. Cell death was assayed in co-cultures of activated microglia (with bacterial LPS) and neurons and in LPS-induced neuroinflammation in mice in vivo. Key results: Obovatol inhibited microglial NO production with an IC50 value of 10 μM. Obovatol also inhibited microglial expression of proinflammatory cytokines and inducible nitric-oxide synthase, which was accompanied by the inhibition of multiple signalling pathways such as nuclear factor kappa B, signal transducers and activators of transcription 1, and mitogen-activated protein kinases. In addition, obovatol protected cultured neurons from microglial toxicity and inhibited neuroinflammation in mice in vivo. One molecular target of obovatol in microglia was peroxiredoxin 2 (Prx2), identified by affinity chromatography and mass spectrometry. Obovatol enhanced the reactive oxygen species (ROS)-scavenging activity of Prx2 in vitro, thereby suppressing proinflammatory signalling pathways of microglia where ROS plays an important role. Conclusions and implications: Obovatol is not only a useful chemical tool that can be used to investigate microglial signalling, but also a promising drug candidate against neuroinflammatory diseases. Furthermore, our results indicate that Prx2 is a novel drug target that can be exploited for the therapeutic modulation of neuroinflammatory signalling.-
dc.publisherWiley-
dc.titleObovatol attenuates microglia-mediated neuroinflammation by modulating redox regulation = 오보바톨에 의한 신경 염증 조절 기전규명-
dc.title.alternativeObovatol attenuates microglia-mediated neuroinflammation by modulating redox regulation-
dc.typeArticle-
dc.citation.titleBritish Journal of Pharmacology-
dc.citation.number8-
dc.citation.endPage1662-
dc.citation.startPage1646-
dc.citation.volume159-
dc.contributor.affiliatedAuthorByoung-Mog Kwon-
dc.contributor.alternativeName옥지연-
dc.contributor.alternativeName한형-
dc.contributor.alternativeName홍수형-
dc.contributor.alternativeName이소연-
dc.contributor.alternativeName한영민-
dc.contributor.alternativeName권병목-
dc.contributor.alternativeName석경호-
dc.identifier.bibliographicCitationBritish Journal of Pharmacology, vol. 159, no. 8, pp. 1646-1662-
dc.identifier.doi10.1111/j.1476-5381.2010.00659.x-
dc.subject.keywordChemical genetics-
dc.subject.keywordMicroglia-
dc.subject.keywordNeuroinflammation-
dc.subject.keywordNeuroprotection-
dc.subject.keywordObovatol-
dc.subject.keywordPeroxiredoxin 2-
dc.subject.localChemical genetics-
dc.subject.localMicroglia-
dc.subject.localNeuroinflammation-
dc.subject.localNeuroprotection-
dc.subject.localObovatol-
dc.subject.localPeroxiredoxin 2-
dc.description.journalClassY-
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Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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