Enhanced biomolecular detection based on localized surface plasmon resonance (LSPR) using enzyme-precipitation reaction

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Title
Enhanced biomolecular detection based on localized surface plasmon resonance (LSPR) using enzyme-precipitation reaction
Author(s)
Seung-Woo Lee; Jun Hyoung Ahn; Min-Gon Kim; Yong Beom Shin; J J Lee; K P Lim; K B Kim
Bibliographic Citation
Journal of Nanoscience and Nanotechnology, vol. 10, no. 5, pp. 3246-3249
Publication Year
2010
Abstract
An enzyme-catalyzed precipitation reaction was employed as a means to increase the change in the LSPR signal after intermolecular bindings between antigens and antibodies occurred on gold nanodot surfaces. The gold nanodot array with an diameter of 175 nm and a thickness of 20 nm was fabricated on a glass wafer using thermal nanoimprint lithography. The human interleukin (hIL) 5 antibody was immobilized on the gold nanodot, followed by binding of hIL 5 to the anti-hIL 5. Subsequently, a biotinylated anti-hIL 5 and a alkaline phosphatase conjugated with streptavidin were simultaneously introduced. A mixture of 5-bromo-4-chloro-3-indolyl phosphate p-toluidine (BCIP) and nitro blue tetrazolium (NBT) was then used for precipitation, which resulted from the biocatalytic reaction of the alkaline phosphatase on gold nanodot. The LSPR spectra were obtained after each binding process. Using this analysis, the enzyme-catalyzed precipitation reaction on gold nanodots was found to be effective in amplifying the change in the peak wavelength of LSPR after molecular bindings.
Keyword
BiosensingEnzyme-Catalyzed precipitationLocalized surface plasmonNanodotNanoimprint lithography (NIL)
ISSN
1533-4880
Publisher
Amer Scientific Publishers
DOI
http://dx.doi.org/10.1166/jnn.2010.2266
Type
Article
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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