A novel WD40 protein, BnSWD1, is involved in salt stress in Brassica napus = 유채의 염 스트레스 관련 유전자 BnSWD1 연구

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Title
A novel WD40 protein, BnSWD1, is involved in salt stress in Brassica napus = 유채의 염 스트레스 관련 유전자 BnSWD1 연구
Author(s)
Sanghun Lee; J Lee; K H Paek; Suk Yoon KwonHye Sun Cho; S J Kim; Jeong Mee Park
Bibliographic Citation
Plant Biotechnology Reports, vol. 4, no. 2, pp. 165-172
Publication Year
2010
Abstract
Genes that are expressed early in specific response to high salinity conditions were isolated from rapeseed plant (Brassica napus L.) using an mRNA differential display method. Five PCR fragments (DD1-5) were isolated that were induced by, but showed different response kinetics to, 200 mM NaCl. Nucleotide sequence analysis and homology search revealed that the deduced amino sequences of three of the five cDNA fragments showed considerable similarity to those of β-mannosidase (DD1), tomato Pti-6 proteins (DD5), and the tobacco harpin-induced protein hin1 (DD4), respectively. In contrast, the remaining clones, DD3 and DD2, did not correspond to any substantial existing annotation. Using the DD3 fragment as a probe, we isolated a full-length cDNA clone from the cDNA library, which we termed BnSWD1 (Brassica napus salt responsive WD40 1). The predicted amino-acid sequence of BnSWD1 contains eight WD40 repeats and is conserved in all eukaryotes. Notably, the BnSWD1 gene is expressed at high levels under salt-stress conditions. Furthermore, we found that BnSWD1 was upregulated after treatment with abscisic acid, salicylic acid, and methyl jasmonate. Our study suggests that BnSWD1, which is a novel WD40 repeat-containing protein, has a function in salt-stress responses in plants, possibly via abscisic acid-dependent and/or -independent signaling pathways.
Keyword
Abscisic acidBrassica napusBrassica napus salt responsive WD40 1genemRNA differential displaySalt stress
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-010-0131-6
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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