Effect of modulation of hnRNP L levels on the decay of bcl-2 mRNA in MCF-7 cells

Cited 5 time in scopus
Metadata Downloads
Title
Effect of modulation of hnRNP L levels on the decay of bcl-2 mRNA in MCF-7 cells
Author(s)
M H Lim; D H Lee; S E Jung; D Y Youn; Chan Sun Park; J H Lee
Bibliographic Citation
Korean Journal of Physiology & Pharmacology, vol. 14, no. 1, pp. 15-20
Publication Year
2010
Abstract
It has been shown that CA repeats in the 3′-untranslated region (UTR) of bcl-2 mRNA contribute the constitutive decay of bcl-2 mRNA and that hnRNP L (heterogenous nuclear ribonucleoprotein L) interacts with CA repeats in the 3′-UTR of bcl-2 mRNA, both in vitro and in vivo. The aim of this study was to determine whether the alteration of hnRNP L affects the stability of bcl-2 mRNA in vivo. Human breast carcinoma MCF-7 cells were transfected with hnRNP L-specific shRNA or hnRNP L-expressing vector to decrease or increase hnRNP L levels, respectively, followed by an actinomycin D chase. An RT-PCR analysis showed that the rate of degradation of endogenous bcl-2 mRNA was not affected by the decrease or increase in the hnRNP L levels. Furthermore, during apoptosis or autophagy, in which bcl-2 expression has been reported to decrease, no difference in the degradation of bcl-2 mRNA was observed between control and hnRNP L-knock down MCF-7 Cells. On the other hand, the levels of AUF-I and nucleolin, transacting factors for ARE in the 3′UTR of bcl-2 mRNA, were not significantly affected by the decrease in hnRNP L, suggesting that a disturbance in the quantitative balance between these transacting factors is not likely to interfere with the effect of hnRNP L. Collectively, the findings indicate that the decay of bcl-2 mRNA does not appear to be directly controlled by hnRNP L in vivo.
Keyword
bcl-2 mRNA stabilityhnRNP L
ISSN
1226-4512
Publisher
South Korea
DOI
http://dx.doi.org/10.4196/kjpp.2010.14.1.15
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.