Effect of modulation of hnRNP L levels on the decay of bcl-2 mRNA in MCF-7 cells

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Effect of modulation of hnRNP L levels on the decay of bcl-2 mRNA in MCF-7 cells
M H Lim; D H Lee; S E Jung; D Y Youn; Chan Sun Park; J H Lee
Bibliographic Citation
Korean Journal of Physiology & Pharmacology, vol. 14, no. 1, pp. 15-20
Publication Year
It has been shown that CA repeats in the 3′-untranslated region (UTR) of bcl-2 mRNA contribute the constitutive decay of bcl-2 mRNA and that hnRNP L (heterogenous nuclear ribonucleoprotein L) interacts with CA repeats in the 3′-UTR of bcl-2 mRNA, both in vitro and in vivo. The aim of this study was to determine whether the alteration of hnRNP L affects the stability of bcl-2 mRNA in vivo. Human breast carcinoma MCF-7 cells were transfected with hnRNP L-specific shRNA or hnRNP L-expressing vector to decrease or increase hnRNP L levels, respectively, followed by an actinomycin D chase. An RT-PCR analysis showed that the rate of degradation of endogenous bcl-2 mRNA was not affected by the decrease or increase in the hnRNP L levels. Furthermore, during apoptosis or autophagy, in which bcl-2 expression has been reported to decrease, no difference in the degradation of bcl-2 mRNA was observed between control and hnRNP L-knock down MCF-7 Cells. On the other hand, the levels of AUF-I and nucleolin, transacting factors for ARE in the 3′UTR of bcl-2 mRNA, were not significantly affected by the decrease in hnRNP L, suggesting that a disturbance in the quantitative balance between these transacting factors is not likely to interfere with the effect of hnRNP L. Collectively, the findings indicate that the decay of bcl-2 mRNA does not appear to be directly controlled by hnRNP L in vivo.
bcl-2 mRNA stabilityhnRNP L
South Korea
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Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
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