Real-time colorimetric screening of endopeptidase inhibitors using adenosine triphosphate (ATP)-stabilized gold nanoparticles = ATP 안정화된 금나노입자를 이용한 효소억제제 실시간 검색

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Title
Real-time colorimetric screening of endopeptidase inhibitors using adenosine triphosphate (ATP)-stabilized gold nanoparticles = ATP 안정화된 금나노입자를 이용한 효소억제제 실시간 검색
Author(s)
M H Kim; S S Lee; Sang Jeon Chung; H H Jang; S Yi; S Kim; S K Chang; M S Han
Bibliographic Citation
Tetrahedron Letters, vol. 51, no. 17, pp. 2228-2231
Publication Year
2010
Abstract
The gold nanoparticles (AuNPs) that were stabilized with adenosine triphosphate (ATP) were stable over a wide range of pHs for the buffer, even in the presence of high concentrations of salt and protein. However, these stabilized AuNPs immediately aggregated when they were exposed to thiol-containing compounds, such as thiophenol. Endoprotease hydrolyzed the thioester bond in the CBZ-Phe-S-Ph substrate, and the hydrolyzed product (thiophenol) reacted with the AuNPs that were stabilized with ATP, causing them to aggregate, which in turn resulted in a visible color change in the AuNPs solution. This method enabled the real-time monitoring of the inhibition potencies of various endopeptidase inhibitors and the activity of endoprotease. This assay discriminated between the inhibition activities of various protease inhibitors for endoprotease on the basis of the color change of the assay solution.
ISSN
0040-4039
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.tetlet.2010.02.061
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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