Signal amplification by enzyme reaction in an immunosensor based on localized surface plasmon resonance (LSPR)

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Title
Signal amplification by enzyme reaction in an immunosensor based on localized surface plasmon resonance (LSPR)
Author(s)
Tae Han Lee; Seung-Woo Lee; Jee Ae Jung; Jun Hyoung Ahn; Min-Gon Kim; Yong Beom Shin
Bibliographic Citation
Sensors, vol. 10, no. 3, pp. 2045-2053
Publication Year
2010
Abstract
An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-γ(IFN-γ) capture antibody was immobilized on the gold NIs, followed by binding of IFN-γ to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecular binding.
Keyword
Enzyme-catalyzed precipitationGold nano-islandImmunosensorLocalized surface plasmon resonance (LSPR)
ISSN
1424-8220
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/s100302045
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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