Time variation of fluorescence lifetime in enhanced cyan fluorescence protein

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Title
Time variation of fluorescence lifetime in enhanced cyan fluorescence protein
Author(s)
S Lee; S Y Kim; Kyoungsook Park; Jin Young Jeong; Bong Hyun Chung; S W Kim
Bibliographic Citation
Journal of Luminescence, vol. 130, no. 7, pp. 1300-1304
Publication Year
2010
Abstract
The lifetime variations of enhanced cyan fluorescence protein (ECFP) in relatively short integration time bins were studied via time-correlated single photon counting (TCSPC) measurement. We observed that minimum photon counts are necessary for the lifetime estimation to achieve a certain range of variance. The conditions to decrease the variance of lifetime were investigated and the channel width of the measurement of TCSPC data was found to be another important factor for the variance of lifetime. Though the lifetime of ECFP is best fit by a double exponential, a mono exponential fit for the same integration time is more stable. The results may be useful in the analysis of photophysical dynamics for ensemble molecules in short measurement time windows.
Keyword
ECFPLifetimeTCSPC
ISSN
0022-2313
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jlumin.2010.02.043
Type
Article
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
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