Phytochemical profiles and in vitro anti-inflammatory properties of Perilla frutescens cv. Chookyoupjaso mutants induced by mutagenesis with γ-ray
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- Phytochemical profiles and in vitro anti-inflammatory properties of Perilla frutescens cv. Chookyoupjaso mutants induced by mutagenesis with γ-ray
- Y D Park; Y M Lee; Min Ah Kang; H J Lee; C H Jin; D S Choi; D S Kim; S Y Kang; W G Kim; I Y Jeong
- Bibliographic Citation
- Food Science and Biotechnology, vol. 19, no. 2, pp. 305-311
- Publication Year
- In earlier investigations, Perilla frutescens (L.) Britt. cv. Chookyoupjaso (CJC) mutants were obtained following mutagenesis induced by 200 Gy of γ-rays. The aim of this study was to compare the CJC and 6 P. frutescens (L.) Britt. cv. Chookyoupjaso mutant lines (CJMs), with respect to their phytochemical profiles and to evaluate anti-inflammatory properties by selecting the most bioactive CJM. The methanol extracts of CJMs were tested for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Among them, CJM-45 showed significant inhibition of NO production. This extract was further partitioned using ethyl acetate (EtOAc), butanol (BuOH), and water. The EtOAc fraction (EF-cjm45) was evaluated for antiinflammatory activities. These results indicated that the EF-cjm45 reduced NO production by inhibiting inducible nitric oxide synthase (iNOS) expression. The EF-cjm45 treatment also significantly diminished expression of MCP-1 and IL-6. In the EF-cjm45, perillaketone, isoegomaketone, ursolic acid, kaempferol, and rosmarinic acid were also found. This study reveals the potential therapeutic use of bioactive CJM-45 and justifies the wide application for this plant in traditional medicine.
- Anti-inflammatorygamma-rayInducible nitric oxide synthase (iNOS)MutantPerilla frutescens (L.) Britt. cv. Chookyoupjaso
- Korea Soc-Assoc-Inst
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