Phytochemical profiles and in vitro anti-inflammatory properties of Perilla frutescens cv. Chookyoupjaso mutants induced by mutagenesis with γ-ray

Cited 7 time in scopus
Metadata Downloads
Title
Phytochemical profiles and in vitro anti-inflammatory properties of Perilla frutescens cv. Chookyoupjaso mutants induced by mutagenesis with γ-ray
Author(s)
Y D Park; Y M Lee; Min Ah Kang; H J Lee; C H Jin; D S Choi; D S Kim; S Y Kang; W G Kim; I Y Jeong
Bibliographic Citation
Food Science and Biotechnology, vol. 19, no. 2, pp. 305-311
Publication Year
2010
Abstract
In earlier investigations, Perilla frutescens (L.) Britt. cv. Chookyoupjaso (CJC) mutants were obtained following mutagenesis induced by 200 Gy of γ-rays. The aim of this study was to compare the CJC and 6 P. frutescens (L.) Britt. cv. Chookyoupjaso mutant lines (CJMs), with respect to their phytochemical profiles and to evaluate anti-inflammatory properties by selecting the most bioactive CJM. The methanol extracts of CJMs were tested for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Among them, CJM-45 showed significant inhibition of NO production. This extract was further partitioned using ethyl acetate (EtOAc), butanol (BuOH), and water. The EtOAc fraction (EF-cjm45) was evaluated for antiinflammatory activities. These results indicated that the EF-cjm45 reduced NO production by inhibiting inducible nitric oxide synthase (iNOS) expression. The EF-cjm45 treatment also significantly diminished expression of MCP-1 and IL-6. In the EF-cjm45, perillaketone, isoegomaketone, ursolic acid, kaempferol, and rosmarinic acid were also found. This study reveals the potential therapeutic use of bioactive CJM-45 and justifies the wide application for this plant in traditional medicine.
Keyword
Anti-inflammatorygamma-rayInducible nitric oxide synthase (iNOS)MutantPerilla frutescens (L.) Britt. cv. Chookyoupjaso
ISSN
1226-7708
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s10068-010-0044-8
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.