DC Field | Value | Language |
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dc.contributor.author | Byung Jo Yu | - |
dc.contributor.author | Jung-Ae Kim | - |
dc.contributor.author | Jae Gu Pan | - |
dc.date.accessioned | 2017-04-19T09:18:41Z | - |
dc.date.available | 2017-04-19T09:18:41Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0305-7453 | - |
dc.identifier.uri | 10.1093/jac/dkq114 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9545 | - |
dc.description.abstract | Objectives: To gain further insight into the defence mechanisms against triclosan in a mutant derived from an Escherichia coli strain carrying the triclosan-resistant target enzyme, FabI(G93V). Methods: An E. coli imp4231 FabI(G93V) strain was constructed by replacing intact fabI with a linear DNA cassette, fabI(G93V)-CmR, that contains a single mutation, GGT to GTT, at codon 93 of fabI(G93V) and a chloramphenicol resistance gene (CmR) as a marker for the mutant allele by a Red-mediated recombination system. Using this E. coli imp4231 FabI(G93V) strain, nitrosoguanidine (NTG) mutagenesis was performed to generate E. coli IFNs [imp4231 FabI(G93V) treated with NTG] displaying higher MICs of triclosan than its parent strain. The genes overexpressed in E. coli IFN4 were identified by DNA microarray analysis. Results: An E. coli imp4231 FabI(G93V) strain displays ~400-fold increased MICs of triclosan (MIC~8 mg/L) compared with the parent strain (MIC~0.02 mg/L). Furthermore, E. coli IFN4 has the highest MIC of triclosan (MIC~80 mg/L). DNA microarray analysis of E. coli IFN4 shows that many genes involved in the biosynthesis of membrane proteins, including transporters, reductases/dehydrogenases and stress response regulators, were highly expressed in the mutant. Conclusions: These results strongly indicate that E. coli IFN cells might protect themselves from triclosan by activating various defence mechanisms, such as (i) changing efflux activities; (ii) capturing the triclosan; and (iii) increasing the expression of important regulators or metabolic enzymes. | - |
dc.publisher | Oxford Univ Press | - |
dc.title | Signature gene expression profile of triclosan-resistant Escherichia coli | - |
dc.title.alternative | Signature gene expression profile of triclosan-resistant Escherichia coli | - |
dc.type | Article | - |
dc.citation.title | Journal of Antimicrobial Chemotherapy | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 1177 | - |
dc.citation.startPage | 1171 | - |
dc.citation.volume | 65 | - |
dc.contributor.affiliatedAuthor | Byung Jo Yu | - |
dc.contributor.affiliatedAuthor | Jung-Ae Kim | - |
dc.contributor.affiliatedAuthor | Jae Gu Pan | - |
dc.contributor.alternativeName | 유병조 | - |
dc.contributor.alternativeName | 김정애 | - |
dc.contributor.alternativeName | 반재구 | - |
dc.identifier.bibliographicCitation | Journal of Antimicrobial Chemotherapy, vol. 65, no. 6, pp. 1171-1177 | - |
dc.identifier.doi | 10.1093/jac/dkq114 | - |
dc.subject.keyword | DNA microarray | - |
dc.subject.keyword | E. coli | - |
dc.subject.keyword | FabI | - |
dc.subject.keyword | Triclosan MIC | - |
dc.subject.local | DNA microarray | - |
dc.subject.local | Escherichia coli. | - |
dc.subject.local | escherichia coli | - |
dc.subject.local | Escherichia Coli | - |
dc.subject.local | Escherichia coli | - |
dc.subject.local | E.coli | - |
dc.subject.local | escherichia coil | - |
dc.subject.local | E. coli | - |
dc.subject.local | E. Coli | - |
dc.subject.local | FabI | - |
dc.subject.local | Triclosan MIC | - |
dc.description.journalClass | Y | - |
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