Signature gene expression profile of triclosan-resistant Escherichia coli

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dc.contributor.authorByung Jo Yu-
dc.contributor.authorJung-Ae Kim-
dc.contributor.authorJae Gu Pan-
dc.description.abstractObjectives: To gain further insight into the defence mechanisms against triclosan in a mutant derived from an Escherichia coli strain carrying the triclosan-resistant target enzyme, FabI(G93V). Methods: An E. coli imp4231 FabI(G93V) strain was constructed by replacing intact fabI with a linear DNA cassette, fabI(G93V)-CmR, that contains a single mutation, GGT to GTT, at codon 93 of fabI(G93V) and a chloramphenicol resistance gene (CmR) as a marker for the mutant allele by a Red-mediated recombination system. Using this E. coli imp4231 FabI(G93V) strain, nitrosoguanidine (NTG) mutagenesis was performed to generate E. coli IFNs [imp4231 FabI(G93V) treated with NTG] displaying higher MICs of triclosan than its parent strain. The genes overexpressed in E. coli IFN4 were identified by DNA microarray analysis. Results: An E. coli imp4231 FabI(G93V) strain displays ~400-fold increased MICs of triclosan (MIC~8 mg/L) compared with the parent strain (MIC~0.02 mg/L). Furthermore, E. coli IFN4 has the highest MIC of triclosan (MIC~80 mg/L). DNA microarray analysis of E. coli IFN4 shows that many genes involved in the biosynthesis of membrane proteins, including transporters, reductases/dehydrogenases and stress response regulators, were highly expressed in the mutant. Conclusions: These results strongly indicate that E. coli IFN cells might protect themselves from triclosan by activating various defence mechanisms, such as (i) changing efflux activities; (ii) capturing the triclosan; and (iii) increasing the expression of important regulators or metabolic enzymes.-
dc.publisherOxford Univ Press-
dc.titleSignature gene expression profile of triclosan-resistant Escherichia coli-
dc.title.alternativeSignature gene expression profile of triclosan-resistant Escherichia coli-
dc.citation.titleJournal of Antimicrobial Chemotherapy-
dc.contributor.affiliatedAuthorByung Jo Yu-
dc.contributor.affiliatedAuthorJung-Ae Kim-
dc.contributor.affiliatedAuthorJae Gu Pan-
dc.identifier.bibliographicCitationJournal of Antimicrobial Chemotherapy, vol. 65, no. 6, pp. 1171-1177-
dc.subject.keywordDNA microarray-
dc.subject.keywordE. coli-
dc.subject.keywordTriclosan MIC-
dc.subject.localDNA microarray-
dc.subject.localEscherichia coli.-
dc.subject.localescherichia coli-
dc.subject.localEscherichia Coli-
dc.subject.localEscherichia coli-
dc.subject.localescherichia coil-
dc.subject.localE. coli-
dc.subject.localE. Coli-
dc.subject.localTriclosan MIC-
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