Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe

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Title
Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe
Author(s)
Dong Uk Kim; J Hayles; D S Kim; V Wood; H O Park; Mi Sun Won; Hyang Sook Yoo; T Duhig; Mi-young Nam; G Palmer; S Han; L Jeffery; S T Baek; H lee; Y S Shim; M Lee; L Kim; K S Heo; E J Noh; A R Lee; Y J Jang; Kyung Sook Chung; S J Choi; J Y Park; Young Woo Park; Hwan Mook Kim; Song Kyu Park; H J Park; E J Kang; H B Kim; H S Kang; H M Park; K Kim; K Song; K B Song; P Nurse; Kwang Lae Hoe
Bibliographic Citation
Nature Biotechnology, vol. 28, no. 6, pp. 617-623
Publication Year
2010
Abstract
We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeastthe only other eukaryote for which a comprehensive knockout library existsrevealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.
ISSN
0733-222X
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/nbt.1628
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Research on National Challenges > 1. Journal Articles
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