Butein sensitizes human hepatoma cells to TRAIL-induced apoptosis via extracellular signal-regulated kinase/Sp1-dependent DR5 upregulation and NF-κB inactivation

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dc.contributor.authorD O Moon-
dc.contributor.authorMun-Ock Kim-
dc.contributor.authorY H Choi-
dc.contributor.authorG Y Kim-
dc.date.accessioned2017-04-19T09:18:53Z-
dc.date.available2017-04-19T09:18:53Z-
dc.date.issued2010-
dc.identifier.issn1535-7163-
dc.identifier.uri10.1158/1535-7163.MCT-09-0942ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9591-
dc.description.abstractTumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces cell death in various types of cancer cells but has little or no effect on normal cells. Human hepatoma cells are resistant to TRAIL-induced apoptosis. Although butein is known to mediate anticancer, anti-inflammatory, and antioxidant activities, little is known about the mechanism of butein in terms of TRAIL-induced apoptosis of human hepatoma cells. In this study, we determined that butein enhances TRAIL-induced apoptosis in hepatoma cells through upregulation of DR5. Luciferase analysis showed that a 5′-flanking region containing four Sp1-binding sites within the DR5 promoter was enhanced by butein (-305/-300). Electrophoretic mobility shift assays and chromatin immunoprecipitation studies were used to analyze the elevation of Sp1 binding to DR5 promoter sites by butein. Point mutations of the Sp1-binding site also attenuated promoter activity. Furthermore, pre-treatment of the blocking chimeric antibody and small interfering RNA for DR5 significantly suppressed TRAIL-mediated apoptosis by butein in Hep3B cells. Butein also stimulated extracellular signal-regulated kinase (ERK) activation, and the ERK inhibitor PD98059 blocked butein-induced DR5 expression and suppressed binding of Sp1 to the DR5 promoter. Additionally, generation of reactive oxygen species had no effect on cell viability, although pretreatment with N-acetyl-L-cysteine or glutathione inhibited combined treatment-induced reactive oxygen species. Indeed, butein repressed the TRAIL-mediated activation of NF-κB and decreased its transcriptional activity. Our results suggest that butein could sensitize certain human hepatoma cells to TRAIL-induced apoptosis through stimulating its death signaling and by repressing the survival function in these cells.-
dc.publisherAmer Assoc Cancer Research-
dc.titleButein sensitizes human hepatoma cells to TRAIL-induced apoptosis via extracellular signal-regulated kinase/Sp1-dependent DR5 upregulation and NF-κB inactivation-
dc.title.alternativeButein sensitizes human hepatoma cells to TRAIL-induced apoptosis via extracellular signal-regulated kinase/Sp1-dependent DR5 upregulation and NF-κB inactivation-
dc.typeArticle-
dc.citation.titleMolecular Cancer Therapeutics-
dc.citation.number6-
dc.citation.endPage1595-
dc.citation.startPage1583-
dc.citation.volume9-
dc.contributor.affiliatedAuthorMun-Ock Kim-
dc.contributor.alternativeName문동오-
dc.contributor.alternativeName김문옥-
dc.contributor.alternativeName최영현-
dc.contributor.alternativeName김기영-
dc.identifier.bibliographicCitationMolecular Cancer Therapeutics, vol. 9, no. 6, pp. 1583-1595-
dc.identifier.doi10.1158/1535-7163.MCT-09-0942-
dc.description.journalClassY-
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Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
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