High frequency plant regeneration system for Nymphoides coreana via somatic embryogenesis from zygotic embryo-derived embryogenic cell suspension cultures

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dc.contributor.authorMyung Jin Oh-
dc.contributor.authorH R Na-
dc.contributor.authorH K Choi-
dc.contributor.authorJang Ryol Liu-
dc.contributor.authorSuk Weon Kim-
dc.date.accessioned2017-04-19T09:18:54Z-
dc.date.available2017-04-19T09:18:54Z-
dc.date.issued2010-
dc.identifier.issn1863-5466-
dc.identifier.uri10.1007/s11816-010-0126-3ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9593-
dc.description.abstractCulture conditions were established for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Nymphoides coreana. Zygotic embryos formed pale-yellow globular structures and calluses at a frequency of 85.6% when cultured on half-strength Murashige and Skoog (MS) medium supplemented with 0.3 mg l-1 of 2,4-D. However, the frequency of pale-yellow globular structures and white callus formation decreased slightly with an increasing concentration of 2,4-D up to 10 mg l-1 with the frequency rate falling to 16.7%. Cell suspension cultures were established from zygotic embryo-derived calluses using half-strength MS medium supplemented with 0.3 mg l-1 of 2,4-D. Upon plating onto half-strength MS basal medium, over 92.3% of cell aggregates gave rise to numerous somatic embryos and developed into plantlets. Regenerated plantlets were successfully transplanted into potting soil and achieved full growth to an adult plant in a growth chamber. The high frequency plant regeneration system for Nymphoides coreana established in this study will be useful for genetic manipulation and cryopreservation of this species.-
dc.publisherSpringer-
dc.titleHigh frequency plant regeneration system for Nymphoides coreana via somatic embryogenesis from zygotic embryo-derived embryogenic cell suspension cultures-
dc.title.alternativeHigh frequency plant regeneration system for Nymphoides coreana via somatic embryogenesis from zygotic embryo-derived embryogenic cell suspension cultures-
dc.typeArticle-
dc.citation.titlePlant Biotechnology Reports-
dc.citation.number2-
dc.citation.endPage128-
dc.citation.startPage125-
dc.citation.volume4-
dc.contributor.affiliatedAuthorMyung Jin Oh-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.affiliatedAuthorSuk Weon Kim-
dc.contributor.alternativeName오명진-
dc.contributor.alternativeName나혜련-
dc.contributor.alternativeName최홍근-
dc.contributor.alternativeName유장렬-
dc.contributor.alternativeName김석원-
dc.identifier.bibliographicCitationPlant Biotechnology Reports, vol. 4, no. 2, pp. 125-128-
dc.identifier.doi10.1007/s11816-010-0126-3-
dc.subject.keywordNymphoides coreana-
dc.subject.keywordPlant regeneration-
dc.subject.keywordSomatic embryogenesis-
dc.subject.keywordZygotic embryo culture-
dc.subject.localNymphoides coreana-
dc.subject.localplant regeneration-
dc.subject.localPlant regeneration-
dc.subject.localsomatic embryogenisis-
dc.subject.localsomatic embryogenesis-
dc.subject.localSomatic embryogenesis-
dc.subject.localzygotic embryo culture-
dc.subject.localZygotic embryo culture-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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