A role for chloroplast-localized Thylakoid formation 1 (THF1) in bacterial speck disease development

Abstract

Coronatine (COR), a jasmonate mimic produced by Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) is required for full virulence of Pst DC3000 in tomato and Arabidopsis. COR is shown to induce a range of physiological processes including chlorosis, root growth inhibition and anthocyanin accumulation in tomato. To elucidate the host/signaling genes involved in CORresponses, we utilized a forward genetics approach using Nicotiana benthamiana and virus-induced gene silencing (VIGS) and identified genes that play a role in COR-mediated chlorosis. We designated these genes as altered COR response (ALC). When silenced, one gene designated ALC1 produced a hypersensitive/necrosis-like phenotype after COR application in a coronatine insensitive 1 (COI1)-dependent manner. In pathogenicity assays performed on Arabidopsis thylakoid formation 1 (thf1) knockout lines and SlALC1-silenced tomato plants, Pst DC3000 induced coalescing necrotic lesions in an accelerated manner. Furthermore, we showed that COR affects ALC1 localization in chloroplast in a COI1-dependent manner. In conclusion, our results show the potential of VIGS-based, forward genetic screens to identify new players in COR-mediated signal transduction.