Whole human exome capture for high-throughput sequencing

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Title
Whole human exome capture for high-throughput sequencing
Author(s)
D W Kim; Seong-Hyeuk Nam; Ryong Nam Kim; Sang-Haeng Choi; Hong-Seog Park
Bibliographic Citation
Genome, vol. 53, pp. 568-574
Publication Year
2010
Abstract
We captured the whole human exome by hybridization using synthesized oligonucleotides, based on a high-density microarray design, and we sequenced those captured human exons using high-throughput sequencing on a Genome Sequencer FLX instrument. of the uniquely mapped reads, 71% fell within target regions, and these corresponded to coverage of 94% of human genes, 87% of exons, and 70% of the total base-pair length of the CCDS set. Our study provides strong evidence for the practical usefulness of this method on a genome-wide scale, showing the resequenced whole human exome database with 501 microRNAs and 307 novel SNPs.
Keyword
exon capturenext-generation sequencingGS FLX sequencer
ISSN
0831-2796
Publisher
Canadian Science Publishing
DOI
http://dx.doi.org/10.1139/G10-025
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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