Sphingopyxis soli sp. nov., isolated from landfill soil

Abstract

A Gram-negative, aerobic, rod-shaped, motile, oxidase-positive, catalase-negative bacterium, designated strain BL03T, was isolated from landfill soil in Pohang, Republic of Korea. Colonies on Luria-Bertani agar plates were yellow. The strain grew in the presence of 0-3% (w/v) NaCl, at 15-42 °C and at pH 5.0-9.5. The predominant ubiquinone was Q-10, and the major cellular fatty acids were C17 : 1ω6c, C15 : 0 2-OH and C18 : 1ω7c. Polar lipids detected were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and an unknown glycolipid. Spermidine was identified as the major polyamine component. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BL03T belongs to the genus Sphingopyxis with high sequence similarity to Sphingopyxis taejonensis JSS54T (97.8 %), Sphingopyxis alaskensis RB2256T (97.4 %) and Sphingopyxis chilensis S37T (96.9 %). Levels of DNA-DNA relatedness between strain BL03T and the above three type strains were only 10.3-40.3 %. The DNA G+C content of strain BL03T was 65.9 mol%. Based on the data presented, strain BL03 T is considered to represent a novel species of the genus Sphingopyxis, for which the name Sphingopyxis soli sp. nov. is proposed. The type strain is BL03T (=KCTC 22405T =JCM 15910T).