Cloning, expression and characterization of xylose isomerase, XylA, from Caldanaerobacter subterraneus subsp. yonseiensis

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dc.contributor.authorByoung Chan Kim-
dc.contributor.authorS N Yu-
dc.contributor.authorK Y Kim-
dc.contributor.authorJung-Sook Lee-
dc.contributor.authorY R Pyun-
dc.contributor.authorS C Ahn-
dc.date.accessioned2017-04-19T09:19:03Z-
dc.date.available2017-04-19T09:19:03Z-
dc.date.issued2010-
dc.identifier.issn0141-5492-
dc.identifier.uri10.1007/s10529-010-0255-3ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9618-
dc.description.abstractThe xylA gene, coding for xylose isomerase, from the extreme thermophile, Caldanaerobacter subterraneus subsp. yonseiensis was cloned, sequenced, and expressed in Escherichia coli. The nucleotide sequence of the xylA gene encoded a polypeptide of 438 residues with a calculated molecular weight of 50,170 Da. The purified XylA showed high sequence homology (92% identity) with that of Thermoanaerobacter thermohydrosulfuricus. The recombinant enzyme expressed in Escherichia coli was purified by heat treatment and gel chromatography. The purified enzyme was thermostable with optimal activity at 95°C. The enzyme required divalent cations including Zn2+ for its maximal activity and thermostability.-
dc.publisherSpringer-
dc.titleCloning, expression and characterization of xylose isomerase, XylA, from Caldanaerobacter subterraneus subsp. yonseiensis-
dc.title.alternativeCloning, expression and characterization of xylose isomerase, XylA, from Caldanaerobacter subterraneus subsp. yonseiensis-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number7-
dc.citation.endPage933-
dc.citation.startPage929-
dc.citation.volume32-
dc.contributor.affiliatedAuthorByoung Chan Kim-
dc.contributor.affiliatedAuthorJung-Sook Lee-
dc.contributor.alternativeName김병찬-
dc.contributor.alternativeName유선영-
dc.contributor.alternativeName김광윤-
dc.contributor.alternativeName이정숙-
dc.contributor.alternativeName변유량-
dc.contributor.alternativeName안순철-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 32, no. 7, pp. 929-933-
dc.identifier.doi10.1007/s10529-010-0255-3-
dc.subject.keywordCaldanaerobacter subterraneus-
dc.subject.keywordThermoanaerobacter-
dc.subject.keywordThermophile-
dc.subject.keywordXylA-
dc.subject.keywordXylose isomerase-
dc.subject.localCaldanaerobacter subterraneus-
dc.subject.localThermoanaerobacter-
dc.subject.localThermophile-
dc.subject.localthermophile-
dc.subject.localThermophiles-
dc.subject.localXylA-
dc.subject.localXylose isomerase-
dc.subject.localxylose isomerase-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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