Dissociation kinetics of singly protonated leucine enkephalin investigated by time-resolved photodissociation tandem mass spectrometry

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dc.contributor.authorJeong Hee Moon-
dc.contributor.authorS H Yoon-
dc.contributor.authorY J Bae-
dc.contributor.authorM S Kim-
dc.date.accessioned2017-04-19T09:19:03Z-
dc.date.available2017-04-19T09:19:03Z-
dc.date.issued2010-
dc.identifier.issn1044-0305-
dc.identifier.uri10.1016/j.jasms.2010.03.025ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9620-
dc.description.abstractThe yields of post-source decay (PSD) and time-resolved photodissociation (PD) at 193 and 266 nm were measured for singly protonated leucine enkephalin ([YGGFL + H]+), a benchmark in the study of peptide ion dissociation, by using tandem time-of-flight mass spectrometry. The peptide ion was generated by matrix-assisted laser desorption ionization (MALDI) using 2,5-dihydroxybenzoic acid as the matrix. The critical energy (E0) and entropy (δS# at 1000 K) for the dissociation were determined by Rice-Ramsperger-Kassel-Marcus fit of the experimental data. MALDI was done for a mixture of YGGFL and Y6 and the plume temperature determined by the kinetic analysis of [Y6 + H]+ data were used to improve the precision of E0 and δS# for [YGGFL + H]+. E0 and δS# thus determined (E0 = 0.67 ± 0.08 eV, δS# = -24.4 ± 3.2 eu with 1 eu = 4.184 J K-1mol-1) were significantly different from those determined by blackbody infrared radiative dissociation (BIRD) (E0 = 1.10 eV, δS# = -14.9 eu), and by surface-induced dissociation (SID) (E0 = 1.13 eV, δS# = -10.3 eu). Analysis of the present experimental data with the SID kinetics (and BIRD kinetics also) led to an unrealistic situation where not only PSD and PD but also MALDI-TOF signals could not be detected. As an explanation for the discrepancy, it was suggested that transition-state switching occurs from an energy bottleneck (SID/BIRD) to an entropy bottleneck (PSD/PD) as the internal energy increases.-
dc.publisherAmer Soc Mass Spectr-
dc.titleDissociation kinetics of singly protonated leucine enkephalin investigated by time-resolved photodissociation tandem mass spectrometry-
dc.title.alternativeDissociation kinetics of singly protonated leucine enkephalin investigated by time-resolved photodissociation tandem mass spectrometry-
dc.typeArticle-
dc.citation.titleJournal of American Society for Mass Spectrometry-
dc.citation.number7-
dc.citation.endPage1158-
dc.citation.startPage1151-
dc.citation.volume21-
dc.contributor.affiliatedAuthorJeong Hee Moon-
dc.contributor.alternativeName문정희-
dc.contributor.alternativeName윤소희-
dc.contributor.alternativeName배용진-
dc.contributor.alternativeName김명수-
dc.identifier.bibliographicCitationJournal of American Society for Mass Spectrometry, vol. 21, no. 7, pp. 1151-1158-
dc.identifier.doi10.1016/j.jasms.2010.03.025-
dc.description.journalClassY-
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Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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