Biosynthesis of medium-chain-length poly(3-hydroxyalkanoates) by volatile aromatic hydrocarbons-degrading Pseudomonas fulva TY16

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Title
Biosynthesis of medium-chain-length poly(3-hydroxyalkanoates) by volatile aromatic hydrocarbons-degrading Pseudomonas fulva TY16
Author(s)
Y Y Ni; Do Young Kim; M G Chung; S H Lee; Ho Yong Park; Y H Rhee
Bibliographic Citation
Bioresource Technology, vol. 101, no. 21, pp. 8485-8488
Publication Year
2010
Abstract
Pseudomonas fulva TY16 biosynthesized medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) containing unsaturated 3-hydroxydodecenoate unit (approximately 8-9%) when grown with volatile aromatic compounds including benzene, toluene, and ethylbenzene as sole carbon substrate. In particular, when cultivated using a continuous feeding system designed to supply toluene at a flow rate of 0.42gL-1h-1 into a 7-L jar fermentor, the growth of the organism reached up to approximately 3.87gL-1 after the 48h fed-batch fermentation, representing an accumulated cellular MCL-PHA of 58.9% by weight. The obtained MCL-PHA was a copolyester primarily consisting of 3-hydroxydecanoate (55.2%) and 3-hydroxyoctanoate (26.8%) with minor constituents being 3-hydroxyhexanoate (3.7%), 3-hydroxydodecenoate (8.2%), and 3-hydroxydodecanoate (6.1%). The present results suggest that P. fulva TY16 is a promising candidate for the biotechnological conversion of toxic petrochemical wastes to valuable biopolymers.
Keyword
Medium-chain-length poly(3-hydroxyalkanoates)PolyhydroxyalkanoatePseudomonas fulva TY16TolueneAromatic hydrocarbons
ISSN
0960-8524
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biortech.2010.06.033
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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