Expression and identification of a minor extracellular fibrinolytic enzyme (Vpr) from Bacillus subtilis KCTC 3014 = Bacillus subtilis KCTC3014 유래 세포 외 분비형 혈전용해 부효소의 동정 및 발현

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dc.contributor.authorNack-Shick Choi-
dc.contributor.authorDong-Min Jeong-
dc.contributor.authorChan Sun Park-
dc.contributor.authorKeug Hyun Ahn-
dc.contributor.authorJoong Su Kim-
dc.contributor.authorJae Jun Song-
dc.contributor.authorSeung Ho Kim-
dc.contributor.authorByung Dae Yoon-
dc.contributor.authorMin-Soo Kim-
dc.date.accessioned2017-04-19T09:19:04Z-
dc.date.available2017-04-19T09:19:04Z-
dc.date.issued2010-
dc.identifier.issn1226-8372-
dc.identifier.uri10.1007/s12257-009-0191-zko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9622-
dc.description.abstractPreviously, three extracellular proteases, Vpr, PepT, and subtilisin were identified from Bacillus subtilis KCTC 3014. To confirm the activity of Vpr, two recombinant Vpr proteins, full Vpr with TTG (pGST-fTTG-Vpr) and full Vpr with ATG (pGST-fATG-Vpr) as an initiation codon were expressed using a pGEX-2T vector encoding glutathione S-transferase (GST) in Escherichia coli. Vpr was produced in two forms, occurring as four spots on a 2- DE gel, 68 and 75 kDa proteins with similar pI values (4.0 ? 4.5). Activity was detected in a fibrin zymography at the expected molecular size of 68 kDa (mature form) processed from full Vpr. However, the recombinant 75 kDa of GST-fVpr did not exhibit activity. Replacement of the TTG codon with ATG led to 1.9-fold increased enzyme activity in 68 kDa. Interestingly, the expression of GSTVpr resulted in the proteolytic degradation of the protein and no GST fusion Vpr protein was detected.-
dc.publisherSpringer-
dc.titleExpression and identification of a minor extracellular fibrinolytic enzyme (Vpr) from Bacillus subtilis KCTC 3014 = Bacillus subtilis KCTC3014 유래 세포 외 분비형 혈전용해 부효소의 동정 및 발현-
dc.title.alternativeExpression and identification of a minor extracellular fibrinolytic enzyme (Vpr) from Bacillus subtilis KCTC 3014-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number3-
dc.citation.endPage452-
dc.citation.startPage446-
dc.citation.volume15-
dc.contributor.affiliatedAuthorNack-Shick Choi-
dc.contributor.affiliatedAuthorDong-Min Jeong-
dc.contributor.affiliatedAuthorChan Sun Park-
dc.contributor.affiliatedAuthorKeug Hyun Ahn-
dc.contributor.affiliatedAuthorJoong Su Kim-
dc.contributor.affiliatedAuthorJae Jun Song-
dc.contributor.affiliatedAuthorSeung Ho Kim-
dc.contributor.affiliatedAuthorByung Dae Yoon-
dc.contributor.affiliatedAuthorMin-Soo Kim-
dc.contributor.alternativeName최낙식-
dc.contributor.alternativeName정동민-
dc.contributor.alternativeName박찬선-
dc.contributor.alternativeName안극현-
dc.contributor.alternativeName김중수-
dc.contributor.alternativeName송재준-
dc.contributor.alternativeName김승호-
dc.contributor.alternativeName윤병대-
dc.contributor.alternativeName김민수-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 15, no. 3, pp. 446-452-
dc.identifier.doi10.1007/s12257-009-0191-z-
dc.subject.keywordBacillus subtilis-
dc.subject.keywordMass spectrometry-
dc.subject.keywordUUG start codon-
dc.subject.keywordVpr-
dc.subject.keywordZymography-
dc.subject.localBacillus subtilis-
dc.subject.localbacillus subtilis-
dc.subject.localMass spetrometry-
dc.subject.localMass spectrometry (MS)-
dc.subject.localmass spectrometry-
dc.subject.localMass spectrometry-
dc.subject.localmass spectrometry (MS)-
dc.subject.localUUG start codon-
dc.subject.localVpr-
dc.subject.localZymography-
dc.subject.localzymography-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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