Purification and characterization of a novel fibrinolytic enzyme from chive (Allium tuberosum)

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dc.contributor.authorDong-Min Jeong-
dc.contributor.authorNack-Shick Choi-
dc.contributor.authorP J Maeng-
dc.contributor.authorHyo Kon Chun-
dc.contributor.authorSeung Ho Kim-
dc.date.accessioned2017-04-19T09:19:07Z-
dc.date.available2017-04-19T09:19:07Z-
dc.date.issued2010-
dc.identifier.issn1226-7708-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9625-
dc.description.abstractA novel Allium tuberosum fibrinolytic enzyme (ATFE) was purified from the leaf of chive by ion exchange chromatography followed by gel filtration. The molecular mass and iso-electric point (pI) of ATFE were 90 kDa and 4.0 by using 1- or 2-D fibrin zymography, respectively. ATFE was optimally active at pH 4.0 and 40°C. ATFE had a high degrading activity for the Aα-chain of human fibrinogen and hydrolyzed the Bβ-chain slowly, but did not affect the γ-chain, indicating that it is a α-fibrinogenase. The proteolytic activity of ATFE was inhibited completely by phenylmethylsulfonyl fluoride (PMSF), indicating that this enzyme belongs to the serine protease class. ATFE was also inhibited by the 1 mM of Cu2+. ATFE exhibited high specificity for Meo-Suc-Arg-Pro-Tyr-pNA (S-2586), a synthetic chromogenic substrate for chymotrypsin. The first 20 amino acid residues of the N-terminal sequence of ATFE were determined as TTKSWNFIGFDETSKXTTYE, which is 60% identical with subtilisin-like serine protease from Narcissus pseudonarcissus. Keywords Allium tuberosum -fibrinolytic enzyme-fibrin zymography-subtilisin-like-
dc.publisherKorea Soc-Assoc-Inst-
dc.titlePurification and characterization of a novel fibrinolytic enzyme from chive (Allium tuberosum)-
dc.title.alternativePurification and characterization of a novel fibrinolytic enzyme from chive (Allium tuberosum)-
dc.typeArticle-
dc.citation.titleFood Science and Biotechnology-
dc.citation.number3-
dc.citation.endPage702-
dc.citation.startPage697-
dc.citation.volume19-
dc.contributor.affiliatedAuthorDong-Min Jeong-
dc.contributor.affiliatedAuthorNack-Shick Choi-
dc.contributor.affiliatedAuthorHyo Kon Chun-
dc.contributor.affiliatedAuthorSeung Ho Kim-
dc.contributor.alternativeName정동민-
dc.contributor.alternativeName최낙식-
dc.contributor.alternativeName맹필재-
dc.contributor.alternativeName전효곤-
dc.contributor.alternativeName김승호-
dc.identifier.bibliographicCitationFood Science and Biotechnology, vol. 19, no. 3, pp. 697-702-
dc.identifier.doi10.1007/s10068-010-0098-7-
dc.subject.keywordAllium tuberosum-
dc.subject.keywordfibrinolytic enzyme-
dc.subject.keywordfibrin zymography-
dc.subject.keywordsubtilisin-like-
dc.subject.localAllium tuberosum-
dc.subject.localFibrinolytic enzyme-
dc.subject.localFibrinolytic enzymes-
dc.subject.localfibrinolytic enzyme-
dc.subject.localFibrin zymography-
dc.subject.localfibrin zymography-
dc.subject.localsubtilisin-like-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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