Expression and characterization of a novel deoxyribose 5-phosp hate aldolase from Paenibacillus sp. EA001

Cited 17 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorY M Kim-
dc.contributor.authorNack-Shick Choi-
dc.contributor.authorY O Kim-
dc.contributor.authorD H Son-
dc.contributor.authorYoung Hyo Chang-
dc.contributor.authorJae Jun Song-
dc.contributor.authorJoong Su Kim-
dc.date.accessioned2017-04-19T09:19:07Z-
dc.date.available2017-04-19T09:19:07Z-
dc.date.issued2010-
dc.identifier.issn1017-7825-
dc.identifier.uri10.4014/jmb.0912.12003ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9626-
dc.description.abstractA novel deoC gene was identified from Paenibacillus sp. EA001 isolated from soil. The gene had an open reading frame (ORF) of 663 base pairs encoding a protein of 220 amino acids with a molecular mass of 24.5 kDa. The amino acid sequence was 79% identical to that of deoxyribose 5-phosphate aldolase (DERA) from Geobacillus sp. Y412MC10. The deoC gene encoding DERA was cloned into an expression vector and the protein was expressed in Escherichia coli. The recombinant DERA was purified using Ni-NTA affinity chromatography and then characterized. The optimum temperature and pH of the enzyme were 50°C and 6.0, respectively. The specific activity for the substrate deoxyribose 5-phosphate (DR5P) was 62 μmol/min/mg. The Km value for DR5P was determined to be 145 mM with the kcat value of 3.2×102/s from Lineweaver-Burk plots. The EA001 DERA showed stability toward a high concentration of acetaldehyde (100 mM).-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleExpression and characterization of a novel deoxyribose 5-phosp hate aldolase from Paenibacillus sp. EA001-
dc.title.alternativeExpression and characterization of a novel deoxyribose 5-phosp hate aldolase from Paenibacillus sp. EA001-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number6-
dc.citation.endPage1000-
dc.citation.startPage995-
dc.citation.volume20-
dc.contributor.affiliatedAuthorNack-Shick Choi-
dc.contributor.affiliatedAuthorYoung Hyo Chang-
dc.contributor.affiliatedAuthorJae Jun Song-
dc.contributor.affiliatedAuthorJoong Su Kim-
dc.contributor.alternativeName김용모-
dc.contributor.alternativeName최낙식-
dc.contributor.alternativeName김용욱-
dc.contributor.alternativeName손동호-
dc.contributor.alternativeName장영효-
dc.contributor.alternativeName송재준-
dc.contributor.alternativeName김중수-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 20, no. 6, pp. 995-1000-
dc.identifier.doi10.4014/jmb.0912.12003-
dc.subject.keywordDeoxyribose 5-phosphate aldolase-
dc.subject.keywordPaenibacillus sp.-
dc.subject.keywordacetaldehyde-
dc.subject.localDeoxyribose 5-phosphate aldolase-
dc.subject.localPaenibacillus sp.-
dc.subject.localPaenibacillus sp-
dc.subject.localpaenibacillus sp.-
dc.subject.localacetaldehyde-
dc.subject.localAcetaldehyde-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Bio-Infrastructure Policy Support Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.